公开(公告)号：EP1351565B1

公开(公告)日：2011-03-16

申请号：EP01901992.6

申请日：2001-01-15

申请人： Morphotek, Inc.

发明人： NICHOLAIDES, Nicholas, C. ,  GRASSO, Luigi ,  SASS, Philip, M.

IPC分类号： A01H1/06 ,  A61K31/7076 ,  A61K31/7088 ,  C12N1/00 ,  C12N5/00 ,  C12Q1/68

CPC分类号： C12N15/1024 ,  A01H3/04 ,  C12N15/01 ,  C12N15/8212 ,  G01N33/5014 ,  G01N33/5088

摘要： Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. Methods of generating mutations in genes of interest and of making various cells mismatch repari defective through the use of chemicals to block mismatch repari in in vivo are disclosed.