公开(公告)号：EP2971130A4

公开(公告)日：2016-10-05

申请号：EP14764629

申请日：2014-03-14

申请人： NUGEN TECHNOLOGIES INC

发明人： AMORESE DOUG ,  SCHROEDER BENJAMIN G ,  SCOLNICK JONATHAN

IPC分类号： C12Q1/68 ,  G06F19/20 ,  G06F19/22

CPC分类号： C12Q1/6874 ,  G06F19/22

摘要： The present invention provides improved methods, compositions and kits for short read next generation sequencing (NGS). The methods, compositions and kits of the present invention enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (typically comprising regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information is obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein are useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.