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    Biochemical oxygen demand analyzer, methods of analysis, microorganisms used for analysis
    1.
    发明公开
    Biochemical oxygen demand analyzer, methods of analysis, microorganisms used for analysis 失效
    生物化学家Sauerstoffbedarfanalysator，Verfahren zur Analyze，Mikroorganismen zur Verwendung in Analyze。

    公开(公告)号：EP0543407A1

    公开(公告)日：1993-05-26

    申请号：EP92119824.8

    申请日：1992-11-20

    申请人： Nakano Vinegar Co., Ltd.

    发明人： Maeda, Shigeru ,  Ohki, Akira ,  Sato, Takeshi ,  Kato, Naho ,  Akano, Hirofumi ,  Kawamura, Yoshiya ,  Hatagaki, Keizo ,  Takahashi, Yasushi ,  Yamada, Mikio ,  Okumura, Hajime

    IPC分类号： C12Q1/00 ,  C12M1/40 ,  C02F3/34 ,  A62D3/00 ,  B09B3/00

    CPC分类号： G01N33/1806 ,  C12Q1/001 ,  G01N33/1866 ,  G01N33/1893 ,  Y10S435/817 ,  Y10S435/852

    摘要： The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in a membrane. Specifically, the present invention relates to a BOD analyzer comprising a flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 µm in diameter by using at least one gelating agent selected from the group consisting of arginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.

    摘要翻译： 本发明涉及一种包含含有氧电极和微生物膜的微生物传感器的BOD分析仪。 微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。 具体而言，本发明涉及一种BOD分析装置，其包括配备有含有氧电极和微生物膜的微生物传感器的流动池; 以及与流通池的入口连接并且具有出口的液体通道。 通过使用至少一种选自藻酸或其盐的凝胶剂，将属于克雷伯氏菌12092菌株的微生物固定在直径为0.65-3μm的平均孔径为0.65-3μm的多孔亲水膜中来制备微生物膜 ，琼脂，结冷胶，黄原胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶和支链淀粉。 本发明还涉及使用BOD分析仪分析BOD的改进方法。