METHOD FOR SEPARATING SUBSTANCES
    3.
    发明公开
    METHOD FOR SEPARATING SUBSTANCES 审中-公开
    VERFAHREN ZUM TRENNEN VON SUBSTANZEN

    公开(公告)号:EP1548429A1

    公开(公告)日:2005-06-29

    申请号:EP03799096.7

    申请日:2003-09-05

    IPC分类号: G01N27/447

    摘要: An objective of the present invention is to provide electrophoretic separation methods and devices that enable the various features of a substrate surface that comes in contact with an electrophoresis medium to be controlled.
    The present invention provides methods for electrophoresing substances, which comprises the steps of:

    (a) adding a substance to be analyzed to an electrophoresis medium retained in a substrate, whose surface that has come in contact with the electrophoresis medium has been coated with a polymer membrane; and
    (b) adding electrophoretic pressure to the electrophoresis medium.

    For example, the use of a plasma-polymerized membrane allows the formation of a membrane with homogeneous quality and thickness on the surface of an arbitrary shape. In addition, desired characteristics can be conferred on the surface through selection of monomeric substances. Protein adsorption onto micro-chips can be effectively prevented as well.

    摘要翻译: 本发明的目的是提供一种电泳分离方法和装置,其能够使与基质表面接触的电泳介质的各种特征得以控制。 本发明提供了电泳物质的方法,其包括以下步骤:(a)将待分析物质加入保留在与电泳介质接触的表面的基质中的电泳介质中 涂有聚合物膜; 和(b)向电泳介质中加入电泳压力。 例如,使用等离子体聚合膜允许在任意形状的表面上形成均匀质量和厚度的膜。 此外,可以通过选择单体物质在表面上赋予所需的特性。 可以有效地防止蛋白质在微芯片上的吸附。