公开(公告)号：EP4282488A1

公开(公告)日：2023-11-29

申请号：EP22759477.7

申请日：2022-02-17

申请人： National University Corporation Kobe University

发明人： TSUJINO Yoshio ,  SATOH Ayano

IPC分类号： A61P37/08 ,  A61P43/00 ,  A61P17/00 ,  A61Q19/08 ,  A61K8/49 ,  C12N15/55 ,  C12N15/63 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6897 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  A61K31/365

摘要： An object of the present invention is to provide a method for easily and efficiently evaluating the ability of test samples to trigger a foreign body reaction in the skin without using animals, a substance having an ability to inhibit a foreign body reaction in the skin, and a screening method for obtaining the substance. The above objects are attained by providing a method for evaluating an ability of a test sample to trigger a foreign body reaction in the skin, comprising: (1) a step of making a solution containing said test sample in contact with a keratinocyte transformed by a reporter vector comprising an enhancer sequence comprising a xenobiotic responsive element (XRE) and a reporter sequence encoding a reporter protein at downstream of said enhancer sequence, (2) a step of measuring an expression level of said reporter protein in said keratinocyte, and (3) a step of evaluating an ability of said test sample to trigger a foreign body reaction in the skin based on the measured expression level of said reporter protein; a method for screening a substance having an ability to inhibit a foreign body reaction in the skin using the evaluation method; and an agent for inhibiting a foreign body reaction in the skin comprising Fraglide-1, which was found using the screening method.