公开(公告)号：EP2692864B1

公开(公告)日：2018-09-12

申请号：EP12765903.5

申请日：2012-03-27

申请人： National University Corporation University Of Toyama

发明人： HORII, Masae ,  KISHI, Hiroyuki ,  KOBAYASHI, Eiji ,  OZAWA, Tatsuhiko ,  MURAGUCHI, Atsushi

IPC分类号： C12N15/63 ,  C12N15/64 ,  C12N15/867 ,  C12N15/86

CPC分类号： C12N15/64 ,  C12N15/63 ,  C12N15/86 ,  C12N15/867 ,  C12N2740/10043 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2820/704 ,  C12N2820/706

摘要： The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D.

公开(公告)号：EP2692864A1

公开(公告)日：2014-02-05

申请号：EP12765903.5

申请日：2012-03-27

申请人： National University Corporation University Of Toyama

发明人： HORII, Masae ,  KISHI, Hiroyuki ,  KOBAYASHI, Eiji ,  OZAWA, Tatsuhiko ,  MURAGUCHI, Atsushi

IPC分类号： C12N15/09

CPC分类号： C12N15/64 ,  C12N15/63 ,  C12N15/86 ,  C12N15/867 ,  C12N2740/10043 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2820/704 ,  C12N2820/706

摘要： The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D.

摘要翻译： 本发明的目的是提供一种装置与它是可以选择的载体高效地哪一个外源基因已被引入。当外源基因是通过同源重组引入到具有同源彼此多个序列的载体。 所述载体包括，连续，复制起点，一个序列A，标记基因X，两个序列C和D，通过同源重组将外源基因，以及与序列A的序列B同源的两个序列C和D是 直接或间接地彼此相邻。 该载体被用于在两个相邻的序列C和D之间将外源基因