公开(公告)号：EP4223860A1

公开(公告)日：2023-08-09

申请号：EP20956146.3

申请日：2020-09-30

申请人： Naturmega S.A.

发明人： MARTÍNEZ GUTIÉRREZ, Wilson ,  PUCHE SIMANCA, Alfredo de Jesús ,  YEPES BUSTILLO, Tatiana Lucía ,  GARCIA PADILLA, Álvaro José ,  BERRIO ROA, Lorena

IPC分类号： C11B1/10 ,  C11B3/02 ,  C11B13/02 ,  C11C1/02 ,  C11C1/08 ,  C11C3/04 ,  C11B3/12

摘要： An aspect of the present invention relates to a method for producing cholesterol from fish oil, which generally comprises the steps of transesterifying refined oil using a basic catalyst; obtaining a stream concentrated in cholesterol and another stream concentrated in omega-3 fatty acids EPA and DHA by means of molecular distillation; and saponifying the cholesterol-rich stream using bases such as potassium hydroxide, calcium hydroxide, sodium hydroxide or calcium chloride. The mixture is subsequently saponified over a range of time and temperature, with agitation and constant reflow. Multiple extractions are carried out using solvents such as toluene, ethylene dichloride, methylene chloride, diethyl ether, petroleum ether, acetone, benzene or a hexane, and centrifugations are performed to obtain the unsaponifiable matter. This last stream is crystallised using acetone, benzene, toluene, diethyl ether, petroleum ether or a hexane to obtain cholesterol with at least 95% purity. In addition, the soap obtained from saponification is transformed into a form of ethyl ester by applying unfolding reactions and acidic esterification.