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    NUCLEIC ACID PROBE SET AND METHOD OF USING THE SAME
    2.
    发明公开
    NUCLEIC ACID PROBE SET AND METHOD OF USING THE SAME 有权
    NUKLEINSÄURESONDENSATZUND VERFAHREN ZUR VERWENDUNG DAVON

    公开(公告)号：EP2319922A1

    公开(公告)日：2011-05-11

    申请号：EP08791869.4

    申请日：2008-07-30

    申请人： Nippon Steel Kankyo Engineering Co., Ltd.

    发明人： ICHIKAWA, Kohei ,  NAKAMURA, Kazunori ,  KURATA, Shinya

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2537/125 ,  C12Q2525/161 ,  C12Q2565/107 ,  C12Q2525/191 ,  C12Q2565/101 ,  C12Q2525/155

    摘要： A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide including (a) a nucleotide labeled with a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b1) a fluorescent probe binding region, to which the fluorescent probe (A) can hybridize, and (b2) a target nucleic acid binding region, which can hybridize to a target nucleic acid (C). The nucleic acid probe set is designed such that, when the fluorescent probe (A) hybridizes to the fluorescent probe binding region (b1) and the target nucleic acid (C) hybridizes to the target nucleic acid binding region (b2), guanine in the target nucleic acid (C) and the fluorescent substance (d) labeled on the nucleotide (a) interact with each other to change a fluorescent character of the fluorescent substance (d). The nucleic acid probe can be furnished without needing preparing a fluorescently-labeled, costly nucleic acid probe specifically for every target nucleic acid.

    摘要翻译： 核酸探针组包括（A）荧光探针和（B）结合探针。 荧光探针（A）由包含（a）用荧光物质标记的核苷酸的寡核苷酸形成。 结合探针（B）由具有（b1）荧光探针结合区域的荧光探针结合区域的荧光探针（A）可以杂交的寡核苷酸形成，（b2）可与靶核酸杂交的靶核酸结合区域 酸（C）。 设计核酸探针组，使得当荧光探针（A）与荧光探针结合区（b1）杂交并且靶核酸（C）与靶核酸结合区（b2）杂交时，鸟嘌呤 靶核酸（C）和核苷酸（a）上标记的荧光物质（d）彼此相互作用以改变荧光物质（d）的荧光特性。 可以提供核酸探针，而不需要为每个靶核酸专门制备荧光标记的昂贵的核酸探针。