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    Thermostable duplex-specific nuclease
    2.
    发明公开
    Thermostable duplex-specific nuclease 有权
    Wärmebeständigeduplexspezifische Nuklease

    公开(公告)号：EP2161331A1

    公开(公告)日：2010-03-10

    申请号：EP09169259.0

    申请日：2009-09-02

    申请人： Nishikawa Rubber Co., Ltd.

    发明人： Hirakawa, Yuzo ,  Ohiso, Isao

    IPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/62 ,  C12Q1/42 ,  C12Q1/68

    CPC分类号： C12N9/22 ,  C12Q1/6813 ,  C12Q2521/301

    摘要： The present invention provides a thermostable duplex-specific nuclease (DSN) and a method for digesting a nucleic acid using said DSN, namely, a protein having a Brachyura-derived duplex-specific nuclease activity, a gene encoding for said protein, a recombinant vector comprising said gene and a transformed cell or transduced cell comprising said vector and a method for producing a protein having a duplex-specific nuclease activity, which comprises culturing said transformed cell or transduced cell using a medium and collecting the protein having a duplex-specific nuclease activity from the cultured mixture, a method for digesting a nucleic acid using said protein having duplex-specific nuclease activity, a method for detecting RNA using said DSN and a reagent kit to be used in the aforementioned methods.

    摘要翻译： 本发明提供了一种热稳定双相特异性核酸酶（DSN）和使用所述DSN消化核酸的方法，即具有Brachyura来源的双链体特异性核酸酶活性的蛋白质，编码所述蛋白质的基因，重组载体 包括所述基因和包含所述载体的转化细胞或转导细胞和用于产生具有双链特异性核酸酶活性的蛋白质的方法，其包括使用培养基培养所述转化细胞或转导细胞并收集具有双链体特异性核酸酶的蛋白质 来自培养的混合物的活性，使用所述具有双链体特异性核酸酶活性的蛋白质消化核酸的方法，使用所述DSN检测RNA的方法和用于上述方法的试剂盒。