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19 条记录 (耗时 0.017 秒)

    Method of producing a nucleic acid
    6.
    发明公开
    Method of producing a nucleic acid 有权
    Verfahren zur Herstellung einerNukleinsäure

    公开(公告)号：EP2138504A1

    公开(公告)日：2009-12-30

    申请号：EP09163543.3

    申请日：2009-06-24

    申请人： Nitto Denko Corporation

    发明人： Maeta, Eri ,  Mori, Kenjiro ,  Konishi, Tatsuya

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00

    摘要： The present invention provides a method of producing a nucleic acid at high reaction efficiency and high reproducibility with a decreased variation in yield and purity among different reaction lots. A nucleic acid synthesis reaction is carried out on a first solid phase carrier capable of supporting nucleic acid synthesis contained in a solid phase carrier mixture comprising the first solid phase carrier and a second solid phase carrier that does not support nucleic acid synthesis.

    摘要翻译： 本发明提供了一种以高反应效率和高再现性制备核酸的方法，其中不同反应批次中产率和纯度的变化减小。 核酸合成反应在能够支持含有第一固相载体和不支持核酸合成的第二固相载体的固相载体混合物中的核酸合成的第一固相载体上进行。

    Production method of porous resin particle having hydroxyl group
    7.
    发明公开
    Production method of porous resin particle having hydroxyl group 有权
    HerstellungsverfahrenfürporöseHarzpartikel mit Hydroxylgruppe

    公开(公告)号：EP2055722A1

    公开(公告)日：2009-05-06

    申请号：EP08019290.9

    申请日：2008-11-04

    申请人： Nitto Denko Corporation

    发明人： Mori, Kenjiro ,  Konishi, Tatsuya

    IPC分类号： C08F2/24 ,  C08F212/06

    CPC分类号： C08F2/20 ,  C08F212/08 ,  C08F212/36 ,  C08F2220/281 ,  C08F2220/302 ,  C08F212/14

    摘要： The present invention relates to a method for producing a porous resin particle containing an aromatic vinyl compound-aromatic divinyl compound copolymer having a hydroxyl group, the method including: dissolving a monomer mixture containing an aromatic vinyl compound, an aromatic divinyl compound and a (meth)acrylic acid ester having one hydroxyl group within the molecule thereof, and a polymerization initiator in an organic solvent to obtain a solution containing the monomer mixture and the polymerization initiator; suspending the solution in water in the presence of a dispersion stabilizer; and performing a suspension copolymerization. The method of the invention is capable of easily producing a porous resin particle containing an aromatic vinyl compound-aromatic divinyl compound copolymer having a hydroxyl group, that is used as a support for solid phase synthesis and enables efficient nucleic acid synthesis.

    摘要翻译： 本发明涉及含有具有羟基的芳香族乙烯基化合物 - 芳香族二乙烯基化合物共聚物的多孔质树脂粒子的制造方法，该方法包括：将含有芳族乙烯基化合物，芳族二乙烯基化合物和（甲基） ）丙烯酸酯和分子内具有一个羟基的聚合引发剂，得到含有单体混合物和聚合引发剂的溶液; 在分散稳定剂的存在下将溶液悬浮在水中; 并进行悬浮共聚。 本发明的方法能够容易地制备含有具有羟基的芳族乙烯基化合物 - 芳族二乙烯基化合物共聚物的多孔树脂颗粒，其用作固相合成的载体并能够有效地进行核酸合成。

    Production method of porous resin bead
    10.
    发明公开
    Production method of porous resin bead 有权
    HerstellungsverfahrenfürporöseHarzperlen

    公开(公告)号：EP2055724A1

    公开(公告)日：2009-05-06

    申请号：EP08019288.3

    申请日：2008-11-04

    申请人： Nitto Denko Corporation

    发明人： Mori, Kenjiro ,  Konishi, Tatsuya ,  Maeta, Eri

    IPC分类号： C08F8/12 ,  C08F212/14 ,  C08F2/18 ,  C08F220/20 ,  C08F212/08

    CPC分类号： C08F6/001 ,  C08F2/18 ,  C08F8/12 ,  C08F8/30 ,  C08F212/08 ,  C08F220/20 ,  C08F2810/50 ,  C08J9/20 ,  C08J2203/12 ,  C08J2203/14 ,  C08J2325/08 ,  C08L25/08 ,  C08F212/14 ,  C08F222/1006 ,  C08F2222/1013 ,  C08F8/14

    摘要： The present invention provides a method for producing a porous resin bead containing an aromatic vinyl compound-hydroxystyrene-di(meth)acrylate copolymer, the method including: dissolving a monomer mixture containing an aromatic vinyl compound, an acyloxystyrene and a di(meth)acrylate compound, and a polymerization initiator in an organic solvent to obtain a solution containing the monomer mixture and the polymerization initiator; suspending the solution in water in the presence of a dispersion stabilizer; performing a suspension copolymerization to thereby obtain an aromatic vinyl compound-acyloxystyrene-di(meth)acrylate compound copolymer; and hydrolyzing an acyloxy group of the acyloxystyrene monomer component in the copolymer. The porous resin bead produced by the method of the present invention can be suitably used, for example, as a support for solid phase synthesis.

    摘要翻译： 本发明提供一种含有芳族乙烯基化合物 - 羟基苯乙烯 - 二（甲基）丙烯酸酯共聚物的多孔树脂珠粒的制造方法，该方法包括：将含有芳族乙烯基化合物，酰氧基苯乙烯和二（甲基）丙烯酸酯的单体混合物 化合物和聚合引发剂，得到含有单体混合物和聚合引发剂的溶液; 在分散稳定剂的存在下将溶液悬浮在水中; 进行悬浮共聚，得到芳香族乙烯基化合物 - 酰氧基苯乙烯 - 二（甲基）丙烯酸酯化合物共聚物; 并在共聚物中水解酰氧基苯乙烯单体组分的酰氧基。 通过本发明的方法制备的多孔树脂珠可以适合用作固相合成的载体。