公开(公告)号：EP1705184A1

公开(公告)日：2006-09-27

申请号：EP06112574.6

申请日：1997-09-17

申请人： Northwestern University

发明人： SOFF, Gerald ,  Gatley, Stephen T. ,  Twardowski, Przemyslaw

IPC分类号： C07K14/00 ,  A61K38/00 ,  C07K14/47 ,  C12N9/68 ,  A61K38/48 ,  C12N9/72

CPC分类号： A61K38/49 ,  A61K38/166 ,  A61K48/00 ,  C12N9/6435 ,  C12Y304/21007 ,  A61K2300/00

摘要： The invention provides methods of generating angiostatin in vitro comprising contacting plasminogen with a plasminogen activator and a sulfhydryl donor or contacting plasmin with a sulfhydryl donor. The invention also provides a method of treating angiogenic diseases by administering to an animal suffering from such a disease a sulfhydryl donor and, optionally, a plasminogen activator, plasminogen or plasmin. The invention further comprises a composition for generating angiostatin comprising a sulfhydryl donor and a plasminogen activator. The invention also provides a container holding a sulfhydryl donor and/or a plasminogen activator, said container having a label thereon instructing administration of the sulfhydryl donor and/or plasminogen activator to an animal suffering from an angiogenic disease. The invention further provides plasminogen fragments-whose N-terminal amino acid is the same as that of plasmin and whose C-terminal amino acid is located in kringle 5 and which inhibit angiogenesis, antibodies which bind selectively to these fragments, methods and kits for using the antibodies, methods and materials for making the fragments by recombinant DNA techniques, and a method of treating an angiogenic disease comprising administering an effective amount of one of the fragments. Finally, the invention provides a method of treating an angiogenic disease comprising administering a transgene coding for one of the fragments.

摘要翻译： 本发明提供了产生在体外血管抑素包括使纤溶酶原与纤溶酶原激活物和巯基供体或具有巯基供体接触纤溶酶的方法。 因此本发明提供了通过从寻求巯供体的疾病和，任选地，纤溶酶原激活剂，纤溶酶原或纤溶酶给予动物治疗患有血管生成性疾病的方法。 本发明还包括用于产生血管抑素包括巯基供体和纤溶酶原激活剂的组合物。 因此本发明提供一种容器保持巯供体和/或纤溶酶原激活剂，在其上具有由在血管发生性疾病指示巯基供体和/或纤溶酶原激活剂的施用给动物的痛苦的标签，所述容器。 本发明进一步提供纤维蛋白溶酶原片段谁的N-末端氨基酸是相同的象纤溶酶和谁的C-末端氨基酸位于三环5和抑制血管生成，选择性结合到合成片段，方法的抗体和试剂盒使用 所述抗体，方法和用于通过重组DNA技术制备的片段的材料，和血管发生性疾病，其包括有效量的片段之一的给药的治疗的方法。 最后，本发明提供了血管生成性疾病，包括施用的转基因编码的片段中的一个的处理的方法。