公开(公告)号：EP2220220A1

公开(公告)日：2010-08-25

申请号：EP08857829.9

申请日：2008-12-02

申请人： Novozymes A/S ,  Abbott Products GmbH

发明人： SVENDSEN, Allan ,  BEIER, Lars ,  LARSEN, Signe, Eskildsen ,  LENHARD, Thomas ,  KJAER, Tanja, Maria, Rosenkilde ,  GREGORY, Peter, Colin

IPC分类号： C12N9/52 ,  C12N15/57 ,  A61K38/48

CPC分类号： C12N9/52 ,  A61K38/00 ,  A61K38/465 ,  A61K38/47 ,  A61K2300/00

摘要： The invention relates to novel variants of a protease derived from Nocardiopsis sp. (SEQ I D NO: 1 ) and closely related proteases, as well as their pharmaceutical use. The variants show improved performance in the treatment of pancreatic exocrine insufficiency (PEI). The variants may be combined with a lipase and/or an amylase. Other examples of medical indications are: Treatment of digestive disorders, pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II.

公开(公告)号：EP2315841A1

公开(公告)日：2011-05-04

申请号：EP09807924.7

申请日：2009-08-14

申请人： Novozymes A/S

发明人： LENHARD, Thomas ,  BJOERNVAD, Mads Eskelund ,  PEDERSEN, Poul Erik

IPC分类号： C12Q1/02 ,  C12Q1/533

CPC分类号： C12Q1/02 ,  C12Q1/533 ,  G01N2333/99

摘要： The invention provides methods of screening a microbial host cell for a property of interest in a microfluidic device, the method comprising the steps of: a) transforming a d-alanine racemase-deficient microbial host cell with a polynucleotide construct comprising: i) one or more polynucleotide region providing the property of interest when present in the host cell, and ii) at least one polynucleotide region complementing the d-alanine racemase deficiency when present in the host cell; and b) screening the transformed host cell for the property of interest in the microfluidic device in the absence of externally provided d-alanine.

公开(公告)号：EP2315841B1

公开(公告)日：2012-08-08

申请号：EP09807924.7

申请日：2009-08-14

申请人： Novozymes A/S

发明人： LENHARD, Thomas ,  BJOERNVAD, Mads Eskelund ,  PEDERSEN, Poul Erik

IPC分类号： C12Q1/02 ,  C12Q1/533

CPC分类号： C12Q1/02 ,  C12Q1/533 ,  G01N2333/99

摘要： The invention provides methods of screening a microbial host cell for a property of interest in a microfluidic device, the method comprising the steps of: a) transforming a d-alanine racemase-deficient microbial host cell with a polynucleotide construct comprising: i) one or more polynucleotide region providing the property of interest when present in the host cell, and ii) at least one polynucleotide region complementing the d-alanine racemase deficiency when present in the host cell; and b) screening the transformed host cell for the property of interest in the microfluidic device in the absence of externally provided d-alanine.