公开(公告)号：EP2831222A4

公开(公告)日：2015-10-21

申请号：EP12872506

申请日：2012-03-27

Applicant: OKINAWA INST OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY

Inventor： DIMITROV DIMITAR IVANOV ,  GUILLAUD LAURENT ELIE ,  TAOUFIQ ZACHARIE MEHDI FRANCOIS ,  TAKAHASHI TOMOYUKI

IPC: C12N1/00 ,  C12N5/0793

CPC classification number: C12N5/0619 ,  C12N5/0618 ,  C12N2500/05 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2502/086

Abstract: An object of the present invention is to develop and provide a method for producing a neuron model having reproduced in vivo properties by improving a cell culture medium composition and a cell culture medium composition necessary for the production of such neuron model. According to the present invention, cell culture is carried out in vitro using a medium for producing in vivo-like and enhanced synaptogenesis neuron model supplemented with a medium supplement for producing in vivo-like and enhanced synaptogenesis neuron model comprising any or any combination of NT-3, potassium or a salt thereof, and FGF2.