公开(公告)号：EP0402108B1

公开(公告)日：1995-02-01

申请号：EP90306131.5

申请日：1990-06-06

申请人： ONCOGEN LIMITED PARTNERSHIP

发明人： Senter, Peter Dana ,  Su, Peter Chong-Dug ,  Marquardt, Hans ,  Hayden, Martha S. ,  Linsley, Peter

IPC分类号： C12N15/55 ,  C12N9/78 ,  C12N1/00

CPC分类号： C12N9/78

公开(公告)号：EP0402108A1

公开(公告)日：1990-12-12

申请号：EP90306131.5

申请日：1990-06-06

申请人： ONCOGEN LIMITED PARTNERSHIP

发明人： Senter, Peter Dana ,  Su, Peter Chong-Dug ,  Marquardt, Hans ,  Hayden, Martha S. ,  Linsley, Peter

IPC分类号： C12N15/55 ,  C12N9/78 ,  C12N1/00

CPC分类号： C12N9/78

摘要： Thermally stable cytosine deaminase (CDase), and the gene coding therefor, is disclosed as well as methods of isolating, purifying, and recombinantly producing the same. The thermally stable CDase can be isolated from Saccharomyces cerevisiae . The yeast isolated enzyme has a molecular weight of approximately 32 kDa, as determined by gel filtration chromatography, and is composed of two subunits, each with a molecular weight of about 17 kDa. Thermally stable yeast CDase so purified shows no significant sequence homology with other known sequenced proteins.

摘要翻译： 公开了热稳定胞嘧啶脱氨酶（CDase）及其编码基因，以及分离，纯化和重组产生其的方法。 可以从酿酒酵母（Saccharomyces cerevisiae）中分离热稳定的CDase。 酵母分离的酶具有通过凝胶过滤层析测定的约32kDa的分子量，由两个分子量约17kDa的亚基组成。 如此纯化的热稳定酵母CDase与其他已知的测序蛋白质没有显着的序列同源性。