公开(公告)号：EP3124973B1

公开(公告)日：2019-01-02

申请号：EP14887513.1

申请日：2014-08-27

申请人： Oilcrops Research Institute of Chinese Academy of Agriculture Sciences

发明人： LI, Peiwu ,  ZHANG, Qi ,  WANG, Yanru ,  ZHANG, Zhaowei ,  DING, Xiaoxia

IPC分类号： G01N33/569 ,  G01N33/544 ,  G01N33/531 ,  C07K16/14

公开(公告)号：EP3124973A1

公开(公告)日：2017-02-01

申请号：EP14887513.1

申请日：2014-08-27

申请人： Oilcrops Research Institute of Chinese Academy of Agriculture Sciences

发明人： LI, Peiwu ,  ZHANG, Qi ,  WANG, Yanru ,  ZHANG, Zhaowei ,  DING, Xiaoxia

IPC分类号： G01N33/569 ,  G01N33/544 ,  G01N33/531

CPC分类号： C07K16/14 ,  C07K1/22 ,  C07K2317/22 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/92 ,  C07K2317/94 ,  G01N33/56961 ,  G01N2333/38

摘要： An aflatoxin nanobody immunoabsorbent and immunoaffinity column and preparation method and use thereof. The immunoabsorbent comprises a solid phase carrier and aflatoxin B1 nanobody 2014AFB-G15 coupled with the solid phase carrier. The 50% inhibiting concentration IC 50 of aflatoxin B1 nanobody 2014AFB-G15 to aflatoxin B1 is 0.66 ng/mL, and the cross-reactivity of aflatoxin B1 nanobody 2014AFB-G15 to aflatoxins B2, G1, G2, and M1 are respectively 22.6%, 10.95%, 32.1% and 26%. The amino acid sequence of aflatoxin B1 nanobody 2014AFB-G15 is as depicted by SEQ ID NO: 7, and the coding gene sequence thereof is as depicted by SEQ ID NO: 8. The aflatoxin nanobody immunoaffinity column can be used for purification and concentration of sample extract prior to computer testing, and the immunoaffinity column can be reused repeatedly.

摘要翻译： 黄曲霉毒素纳米体免疫吸附和免疫亲和柱和制备方法及其用途。 在免疫吸附包括固相载体和黄曲霉毒素B1的纳米体2014AFB-G15加上固相载体。 50％抑制浓度的黄曲霉毒素B1的纳米体2014AFB-G15的IC 50至黄曲霉毒素B1为0.66毫微克/毫升，和黄曲霉毒素B1的纳米体2014AFB-G15的交叉反应黄曲霉毒素B2，G1，G2和M1分别为22.6％ 10.95％，32.1％和26％。 黄曲霉毒素B1的纳米体2014AFB-G15的氨基酸序列如所描绘的SEQ ID NO：7，以及它们的如SEQ ID NO所示的编码基因序列：8.黄曲霉毒素纳米体免疫亲和柱可用于纯化和浓度 前计算机测试样品提取物和免疫亲和柱可反复再使用。

公开(公告)号：EP3124498A1

公开(公告)日：2017-02-01

申请号：EP14887280.7

申请日：2014-08-27

申请人： Oilcrops Research Institute of Chinese Academy of Agriculture Sciences

发明人： LI, Peiwu ,  WANG, Yanru ,  ZHANG, Qi ,  ZHANG, Zhaowei ,  DING, Xiaoxia

IPC分类号： C07K16/14 ,  G01N33/53

CPC分类号： C07K16/14 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2317/92 ,  C07K2317/94

摘要： Disclosed is aflatoxin B1 nanobody 2014AFB-G15. An amino acid sequence thereof is shown in SEQ ID NO: 7, and a gene coding sequence thereof is shown in SEQ ID NO: 8. Aflatoxin B1 nanobody 2014AFB-G15 obtained in the present disclosure has the properties of resistance to organic reagents, resistance to high temperature, and good stability. 50% inhibitory concentration IC 50 of aflatoxin B1 nanobody 2014AFB-G15 against aflatoxin B1 is 0.66 ng/mL, and cross-reaction rates thereof with aflatoxin B2, aflatoxin G1, aflatoxin G2, and aflatoxin M1 are 22.6%, 0.95%, 32.1%, and 26%, respectively.

摘要翻译： 披露了黄曲霉毒素B1纳米颗粒2014AFB-G15。 其氨基酸序列示于SEQ ID NO：7，其基因编码序列如SEQ ID NO：8所示。本公开获得的黄曲霉毒素B1纳米颗粒2014AFB-G15具有耐有机试剂，耐药性 耐高温，稳定性好。 黄曲霉毒素B1纳米颗粒对AF黄曲霉毒素B1的50％抑制浓度IC 50为0.66ng / mL，黄曲霉毒素B2，黄曲霉毒素G1，黄曲霉毒素G2和黄曲霉毒素M1的交叉反应率分别为22.6％，0.95％，32.1％ ，26％。