公开(公告)号：EP2110433A1

公开(公告)日：2009-10-21

申请号：EP08710648.0

申请日：2008-01-30

申请人： Oriental Yeast Co., Ltd. ,  Nissin Foods Holdings Co., Ltd.

发明人： YANO, Takeo ,  SAKAI, Yumiko ,  UCHIDA, Kouji ,  NAKAO, Yoshiki ,  ISHIHATA, Kimie ,  NAKANO, Shigeru ,  YAMADA, Toshihiro

IPC分类号： C12N15/00 ,  C12N15/09 ,  C12Q1/68

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C07K14/415

摘要： [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut.
[Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.

摘要翻译： [对象]新开发一种核桃可以简单，快速，准确地分离和区别于山核桃坚果的方法。 [解决方法]由于核桃过敏原患者数量的增加以及日本最近在日本的菊科植物中属于可食用坚果的山核桃进口量的增加，因此开发了检测核桃的方法 迫切需要有歧视的手段。 重点关注具有表观基因序列的叶绿体matK基因，我们开发了一种特异性检测菊科植物坚果的PCR方法，并鉴定了山核桃坚果的matK序列，建立了一种用核桃和山核桃果实进行限制的方法 用于区分核桃和山核桃果仁的酶。