专利检索 ap:("PANORAMA RESEARCH, INC.") AND inv:"LARRICK, James W." 第 1 页

1.

发明公开
A 24 KILODALTON CYTOPLASMIC PROTEASE ACTIVATING DNA FRAGMENTATION APOPTOSIS 失效
标题翻译：凋亡的DNA片段化活化蛋白酶24kDa的细胞质

公开(公告)号：EP0767799A1

公开(公告)日：1997-04-16

申请号：EP95922207.0

申请日：1995-06-05

申请人： PANORAMA RESEARCH, INC.

发明人： WRIGHT, Susan C. , LARRICK, James W.

IPC分类号： C12N9 , A61K38

CPC分类号： C12N9/6421 , A61K38/00 , Y10S514/01

摘要： A 24 kilodalton cytoplasmic protease which cleaves at proline-valine bonds is purified from UV-induced human U937 histiocytic lymphoma cells by affinity chromatography using the inhibitor DK120 as the ligand, followed by heparin-sepharose chromatography and reverse phase HPLC. Treating nuclei of uninduced U937 cells with the affinity-purified protease is sufficient to activate internucleosomal DNA fragmentation characteristic of apoptosis as demonstrated by gel electrophoresis in the figure.