公开(公告)号：EP1564554A1

公开(公告)日：2005-08-17

申请号：EP04075439.2

申请日：2004-02-12

申请人： PEPSCAN SYSTEMS B.V. ,  Universiteit Utrecht Holding B.V.

发明人： Akresteijn, Gerardus Josephus Adrianus ,  Hensen, Everard Johannes ,  Scibelli, Antonio ,  van der Most, Robbert Gerrit ,  Meloen, Robert Hans ,  Turkstra, Jouwert Anne

IPC分类号： G01N33/50

CPC分类号： G01N33/56972 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54393

摘要： The present invention discloses an affinity-binding assay comprising a particle having at least four copies of a target molecule and at least two binding molecules specific for said target molecule, wherein a first of said binding molecules is associated with a first label and a second of said binding molecules is associated with a second label, wherein a particle having said first label and a particle having said second label are distinguishable from a particle having both said first and said second label, wherein said first and said second binding molecule each comprise at least two binding regions specific for said target molecule.
The present invention also discloses a composition comprising a first binding molecule associated with a first label and a second binding molecule associated with a second label, wherein the signal obtained from said first label and said second label is distinguishable from the combined signal of said first and second label, characterised in that said first and said second binding molecule each comprise at least two binding regions specific for essentially the same target molecule, preferably for essentially the same epitope on said target molecule and a method for selecting a synthetic antigen from a collection of at least three antigens comprising using the disclosed composition and method.
The invention further discloses an antigen obtainable by above described method and capable of inducing an early immune response, a kit of parts to perform said method, the use of antigens selected by said methods for use as a vaccine, and the use of antibodies and antigens as a medicament.

摘要翻译： 本发明公开了一种亲和力结合测定法，其包含具有至少四个拷贝的靶分子和至少两个对所述靶分子特异性的结合分子的颗粒，其中第一个所述结合分子与第一个标记相关联，第二个 所述结合分子与第二标记相关联，其中具有所述第一标记的颗粒和具有所述第二标记的颗粒与具有所述第一和所述第二标记两者的颗粒可区分，其中所述第一和所述第二结合分子各自包含至少 对于所述靶分子具有特异性的两个结合区。 本发明还公开了一种组合物，其包含与第一标记相关联的第一结合分子和与第二标记相关联的第二结合分子，其中从所述第一标记和所述第二标记获得的信号与组合信号区分开 的所述第一和第二标记，其特征在于所述第一和所述第二结合分子各自包含至少两个对基本相同的靶分子特异性的结合区，优选用于所述靶分子上基本相同的表位，以及选择合成抗原的方法 包括使用所公开的组合物和方法的至少三种抗原的集合。 本发明还公开了可通过上述方法获得的能够诱导早期免疫应答的抗原，用于实施所述方法的试剂盒，所述方法选择的抗原用作疫苗，以及使用 的抗体和抗原作为药物。