专利检索 ap:("POSTECH FOUNDATION" OR "POSCO" OR "POSTECH ACAD IND FOUND") AND inv:"SEO JONG-CHEOL" 第 1 页

1.

发明公开
METHOD OF DE NOVO SEQUENCING OF PEPTIDE USING THE MALDI MASS SPECTROMETRY, METHOD AND APPARATUS FOR PREPARING SAMPLE FOR MALDI MASS SPECTROMETRY 有权
标题翻译：方法从头肽测序与MALDI质谱分析，其制造用探头的MALDI质谱分析方法和装置

公开(公告)号：EP1871898A4

公开(公告)日：2009-04-15

申请号：EP06716271

申请日：2006-03-08

申请人： POSTECH FOUNDATION , POSCO , POSTECH ACAD IND FOUND

发明人： SHIN SEUNG-KOO , JEONG KYUNG-HWAN , YOON HYE-JOO , SUH MIN-SOO , SEO JONG-CHEOL

IPC分类号： C12Q1/68

CPC分类号： G01N33/6851

摘要： The present invention relates to a method of peptide sequencing by MALDI (matrix-assisted laser desorption ionization) tandem mass spectrometry, which comprises the steps of chemically modifying a sample peptide with at least one chemical modification method selected from the group consisting of guanidination and esterification in order to change the ionization status of the peptide and performing mass spectrometry using a MALDI tandem mass spectrometer and programmed MALDI tandem mass spectrometry. The peptide sequencing method of the present invention is advantageous in that detection sensitivity of the peptide improves significantly and various daughter ions are detected uniformly, thereby enabling perfect de novo sequencing with tandem mass spectrometry only, without database search.