公开(公告)号：EP3744727A1

公开(公告)日：2020-12-02

申请号：EP20174779.7

申请日：2014-03-14

申请人： Pioneer Hi-Bred International, Inc. ,  E. I. du Pont de Nemours and Company

发明人： HU, Xu ,  PRESNAIL, James ,  RICHTMAN, Nina ,  DIEHN, Scott ,  VAN ALLEN, Michelle ,  PROCYK, Lisa

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N5/04 ,  C12N5/10 ,  C12N15/00

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

公开(公告)号：EP2970363B1

公开(公告)日：2020-07-08

申请号：EP14767960.9

申请日：2014-03-14

申请人： Pioneer Hi-Bred International, Inc. ,  E. I. du Pont de Nemours and Company

发明人： HU, Xu ,  PRESNAIL, James ,  RICHTMAN, Nina ,  DIEHN, Scott ,  VAN ALLEN, Michelle ,  PROCYK, Lisa

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N5/04 ,  C12N5/10 ,  C12N15/00

公开(公告)号：EP2970363A2

公开(公告)日：2016-01-20

申请号：EP14767960.9

申请日：2014-03-14

申请人： Pioneer Hi-Bred International, Inc. ,  E. I. du Pont de Nemours and Company

发明人： HU, Xu ,  PRESNAIL, James ,  RICHTMAN, Nina ,  DIEHN, Scott ,  VAN ALLEN, Michelle ,  PROCYK, Lisa

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12N5/04 ,  C12N5/10 ,  C12N15/00

摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

摘要翻译： 提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物例如鞘翅目植物害虫或Diabrotica植物害虫摄入时降低害虫中靶序列的表达。 本发明提供了本文公开的任何SEQ ID NO：（但不包括正向和反向引物）或其活性变体和片段或其互补物中列出的各种靶多核苷酸，其中一个或多个 目标害虫中的更多序列控制害虫（即具有杀虫活性）。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。