公开(公告)号：EP2027276B1

公开(公告)日：2011-02-02

申请号：EP07845231.5

申请日：2007-05-15

申请人： Pioneer Hi-Bred International, Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY ,  The Regents of the University of California

发明人： ALTIER, Daniel, J. ,  CRANE, Virginia, C. ,  GILLIAM, Jacob, T. ,  HUNTER-CEVERA, Jennie ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLANSKAYA, I.A.

IPC分类号： C12N15/82 ,  C07K14/385

CPC分类号： C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

公开(公告)号：EP2027276A2

公开(公告)日：2009-02-25

申请号：EP07845231.5

申请日：2007-05-15

申请人： Pioneer Hi-Bred International Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY ,  The Regents of the University of California

发明人： ALTIER, Daniel, J. ,  CRANE, Virginia, C. ,  GILLIAM, Jacob, T. ,  HUNTER-CEVERA, Jennie ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLANSKAYA, I.A.

IPC分类号： C12N15/82 ,  C07K14/385

CPC分类号： C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

公开(公告)号：EP1056862A1

公开(公告)日：2000-12-06

申请号：EP99906944.6

申请日：1999-02-11

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： CRANE, Virginia, C.

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/10 ,  C07K14/415 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8279

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for inducible and constitutive promoters isolated from a family of maize genes encoding pathogenesis-related (PR-1) proteins, nucleotide sequences for these maize PR-1 genes, and amino acid sequences for the PR-1 proteins encoded thereby. Methods for regulating expression of a heterologous nucleotide sequence in a plant in an inducible or constitutive manner are provided, more particularly regulating expression of a PR-1 gene to enhance disease resistance in a plant. The methods comprise transforming a plant cell to comprise a heterologous nucleotide sequence or PR-1 gene operably linked to one of the inducible or constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell. Transformed plants and seeds having enhanced disease resistance are also provided.