公开(公告)号：EP3464293A1

公开(公告)日：2019-04-10

申请号：EP17803405.4

申请日：2017-05-23

申请人： Portland State University

发明人： STRONGIN, Robert, M. ,  SIBRIAN-VAZQUEZ, Martha ,  WANG, Lei ,  ESCOBEDO CORDOVA, Jorge, O. ,  LOWRY, Mark, A.

IPC分类号： C07D491/16 ,  C07D311/82 ,  G01N21/64 ,  G01N33/483 ,  C09B11/04

公开(公告)号：EP2970325A2

公开(公告)日：2016-01-20

申请号：EP14765163.2

申请日：2014-03-14

申请人： Portland State University

发明人： STRONGIN, Robert ,  SIBRIAN-VAZQUEZ, Martha ,  WANG, Jialu ,  WANG, Lei ,  ESCOBEDO CORDOVA, Jorge, O.

IPC分类号： C07D491/107 ,  C07D491/22 ,  G01N21/64 ,  G01N33/52 ,  G01N33/92 ,  C07B63/00 ,  C09K11/06

CPC分类号： G01N33/92 ,  C07D491/107 ,  C07D491/22 ,  C09B11/24

摘要： Embodiments of methods and compounds for isolating and detecting lysophosphatidic acids (LPAs) are disclosed. Kits for performing the methods also are disclosed. LPAs are isolated from biological samples by liquid-liquid extraction followed by solid phase extraction. LPA species may be separated by HPLC, and the separated species may be identified and quantified. Also disclosed are embodiments of compounds capable of universally detecting a plurality of LPA species with substantially equivalent sensitivity. Embodiments of the disclosed compounds are useful for determination of total LPA concentration in a sample comprising a plurality of LPA species without separation of individual LPA species.

摘要翻译： 公开了用于分离和检测溶血磷脂酸（LPA）的方法和化合物的实施方案。 还公开了用于执行该方法的试剂盒。 通过液 - 液萃取然后固相萃取将LPA从生物样品中分离出来。 LPA物种可以通过HPLC分离，并且可以鉴定和定量分离的物种。 还公开了能够以基本相等的灵敏度普遍检测多种LPA物质的化合物的实施方案。 公开的化合物的实施方式可用于确定包含多种LPA物质的样品中的总LPA浓度，而不分离单独的LPA物质。

公开(公告)号：EP2970325B1

公开(公告)日：2017-09-27

申请号：EP14765163.2

申请日：2014-03-14

申请人： Portland State University

发明人： STRONGIN, Robert ,  SIBRIAN-VAZQUEZ, Martha ,  WANG, Jialu ,  WANG, Lei ,  ESCOBEDO CORDOVA, Jorge, O.

IPC分类号： C07D491/107 ,  C07D491/22 ,  G01N21/64 ,  G01N33/52 ,  G01N33/92 ,  C07B63/00 ,  C09K11/06

CPC分类号： G01N33/92 ,  C07D491/107 ,  C07D491/22 ,  C09B11/24

摘要： Embodiments of methods and compounds for isolating and detecting lysophosphatidic acids (LPAs) are disclosed. Kits for performing the methods also are disclosed. LPAs are isolated from biological samples by liquid-liquid extraction followed by solid phase extraction. LPA species may be separated by HPLC, and the separated species may be identified and quantified. Also disclosed are embodiments of compounds capable of universally detecting a plurality of LPA species with substantially equivalent sensitivity. Embodiments of the disclosed compounds are useful for determination of total LPA concentration in a sample comprising a plurality of LPA species without separation of individual LPA species.