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1.发明公开
公开(公告)号：EP4403626A1
公开(公告)日：2024-07-24
申请号：EP21957266.6
申请日：2021-10-26
发明人： LIU, Qingsong , HU, Jie , CHEN, Cheng , HUANG, Tao
摘要： A primary cell culture medium for culturing primary lung cancer epithelial cells, a culture method using the primary cell culture medium, and an application thereof in drug efficacy evaluation and screening. The culture medium contains an MST1/2 kinase inhibitor, a ROCK kinase inhibitor, a fibroblast growth factor, at least one additive selected from a B27 additive and an N2 additive, an epidermal growth factor, transferrin, gastrin, and a TGFβ type I receptor inhibitor.
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2.发明公开
公开(公告)号：EP4137565A1
公开(公告)日：2023-02-22
申请号：EP20930874.1
申请日：2020-04-23
发明人： LIU, Qingsong , HU, Jie , WANG, Wenchao , CHEN, Cheng , REN, Tao , WANG, Li
摘要： Provided are a primary cell culture medium that contains a combination of an MST1/2 kinase inhibitor and a ROCK kinase inhibitor and is used for culturing primary esophageal squamous cell carcinoma epithelial cells, and a culture method using the primay cell culture medium. In the culture method, the primary cell culture medium is used to culture primay cells on a culture vessel coated with an extracellular matrix glue, so that the primary cells prolilferate rapidly. A cell model obtained by using the primary cell culture medium and the primary cell culture method of the present invention can be used for the efficacy evaluation and screening of drugs.
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3.发明公开
公开(公告)号：EP4368706A1
公开(公告)日：2024-05-15
申请号：EP21948946.5
申请日：2021-07-28
发明人： LIU, Qing Song , HU, Jie , HUANG, Tao , CHEN, Cheng
摘要： A primay cell culture medium for culturing primary lung cancer epithelial cells, containing an MST1/2 kinase inhibitor, a ROCK inhibitor, fibroblast growth factor 7, at least one additive of B27 additive and N2 additive, hepatocyte growth factor, insulin-like growth factor 1, interleukin 6, and a TGFβ type I receptor inhibitor. The invention also relates to a culture method using the primary cell culture medium and its application in the efficacy evaluating and screening of drugs. In the culture method, the primary cell culture medium is used to culture primay cells in a culture vessel coated with an extracellular matrix gel, to make the primary cells expend rapidly. A cell model obtained using the primary cell culture medium and the primary cell culture method can be used for the efficacy evaluating and screening of drugs.
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4.发明公开
公开(公告)号：EP4105320A1
公开(公告)日：2022-12-21
申请号：EP20918987.7
申请日：2020-02-26
发明人： LIU, Qingsong , HU, Jie , CHEN, Cheng , WANG, Wenchao , WANG, Li
IPC分类号： C12N5/09
摘要： Provided are a culture medium and cultivation method of rapidly expanding primary cells of esophageal squamous carcinoma in vitro, and the use thereof in screening drugs. The culture medium comprises an initial culture medium selected from DMEM/F12, DMEM, F12, or RPMI-1640, a Rho protease inhibitor, an antibiotic, insulin, an N2 additive, insulin-like growth factor 1, a non-essential amino acid, and optionally, hydrocortisone, optionally, glutamine, and optionally, bovine pituitary extract.
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