公开(公告)号：EP3202909A4

公开(公告)日：2018-05-23

申请号：EP15847264

申请日：2015-07-23

申请人： RZNOMICS INC

发明人： LEE SEONG WOOK ,  KIM JU HYUN ,  JEONG JIN SOOK ,  HAN SANG YOUNG

IPC分类号： C12N15/85 ,  A61K31/713 ,  A61K38/43 ,  A61P35/00 ,  C12N9/22

CPC分类号： A61K31/713 ,  A61K38/00 ,  A61K38/43 ,  C12N15/1135 ,  C12N15/85 ,  C12N15/86 ,  C12N2310/113 ,  C12N2310/124 ,  C12N2330/51 ,  C12N2710/10343 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/48

摘要： The present invention relates to a recombination vector, a transformation cell into which the recombinant vector is introduced, a ribozyme expressed from the recombination vector, a prophylactic or therapeutic composition for liver cancer comprising the recombination vector and the ribozyme, and a therapeutic method for liver cancer using the composition, said recombination vector comprising: a tissue-specific promoter; and a ribozyme-target gene expression cassette comprising a trans-splicing ribozyme targeting a cancer-specific gene and a target gene connected to the 3' exon of the ribozyme, wherein a splicing donor/splicing acceptor sequence (SD/SA sequence) is connected to the 5' end of the ribozyme-target gene expression cassette, woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is connected to the 3' end of the ribozyme-target gene expression cassette, and a nucleic acid sequence recognizing a micro RNA-122a (microRNA-122a, miR-122a) is further connected to the 3' end of the WPRE.