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公开(公告)号：EP1431387A1
公开(公告)日：2004-06-23
申请号：EP03028861.7
申请日：2003-12-16
发明人： Mueller, Rainer, Dr. , Kirschbaum, Thomas, Dr. , Suppmann, Bernhard, Dr. , Schoen, Helmut, Dr. , Engh, Richard, Dr. , Hoffmann, Artur, Dr. , Thalhofer, Johan-Peter, Dr. , Siedel, Joachim, Dr. , Engel, Wolf-Dieter, Dr.
CPC分类号： C12N9/22 , C12Y301/21001
摘要： According to the invention, the desoxyribonuclease with increased thermolability is a variant, by way of amino acid substitution, of bovine pancreatic desoxyribonuclease I. The variant protein retains desoxyribonuclease activity. Moreover, the specific desoxyribonuclease activity of the variant of bovine pancreatic desoxyribonuclease I is approximately zero units per mg of protein following heating of the variant of bovine pancreatic desoxyribonuclease I for about 5 min at a temperature below 95°C. In addition, the variant of bovine pancreatic desoxyribonuclease I has no measurable ribonuclease activity.
摘要翻译： 根据本发明，具有增加的热稳定性的脱氧核糖核酸酶是通过氨基酸取代的牛胰酶脱氧核糖核酸酶I的变体。变体蛋白保留脱氧核糖核酸酶活性。 此外，牛胰酶脱氧核糖核酸酶I的变体的特异性脱氧核糖核酸酶活性在牛胰蛋白脱氧核糖核酸酶I的变体在95℃以下的温度下加热约5分钟后，每毫克蛋白质大约为零单位。另外， 牛胰腺脱氧核糖核酸酶我没有可测量的核糖核酸酶活性。
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公开(公告)号：EP1431387B1
公开(公告)日：2008-02-27
申请号：EP03028861.7
申请日：2003-12-16
发明人： Mueller, Rainer, Dr. , Kirschbaum, Thomas, Dr. , Suppmann, Bernhard, Dr. , Schoen, Helmut, Dr. , Engh, Richard, Dr. , Hoffmann, Artur, Dr. , Thalhofer, Johan-Peter, Dr. , Siedel, Joachim, Dr. , Engel, Wolf-Dieter, Dr.
CPC分类号： C12N9/22 , C12Y301/21001
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