公开(公告)号：EP4316840A1

公开(公告)日：2024-02-07

申请号：EP22306178.9

申请日：2022-08-03

申请人： SARTORIUS STEDIM FMT SAS ,  UNIVERSITE CLAUDE BERNARD - LYON 1 ,  Ecole Superieure de Chimie, Physique, Electronique de Lyon ,  Institut National des Sciences Appliquees de Lyon (Insa Lyon) ,  Centre National de la Recherche Scientifique (CNRS)

发明人： Gay, Isabelle ,  Barbaroux, Magali ,  Dufour, Alexandre ,  Marquette, Christophe ,  Petiot, Emma

IPC分类号： B33Y70/00 ,  B29C64/106 ,  C12M1/00 ,  C12M3/00 ,  C12M1/26

摘要： The invention concerns a sterile enclosure (2) and a method of printing and culturing a three-dimensional biological structure in the sterile enclosure. The sterile enclosure comprising:
- a base wall (10),
- a flexible side wall (8); the side wall forming with the base wall (10) a flexible casing (12) delimiting an interior chamber (14),
- a fluid inlet port (22) and a fluid outlet port (24) which allow the circulation of a culture media,
- a needle adapter (4) fixed to the flexible side wall (8), the needle adapter (4) holding a printing needle (6),
the flexible casing protecting a biological three-dimensional structure against contamination and authorizing at the same time the move of the needle adapter (4) in three directions during a printing of a biological three-dimensional structure in the interior chamber.

公开(公告)号：EP4316843A1

公开(公告)日：2024-02-07

申请号：EP22306181.3

申请日：2022-08-03

申请人： SARTORIUS STEDIM FMT SAS ,  UNIVERSITE CLAUDE BERNARD - LYON 1 ,  Institut National des Sciences Appliquees de Lyon (Insa Lyon) ,  Centre National de la Recherche Scientifique (CNRS) ,  Ecole Superieure de Chimie, Physique, Electronique de Lyon

发明人： Gay, Isabelle ,  Barbaroux, Magali ,  Dufour, Alexandre ,  Marquette, Christophe ,  Petiot, Emma

IPC分类号： B33Y70/00 ,  B29C64/106 ,  C12M1/26 ,  C12M1/00 ,  C12M3/00

摘要： A bioreactor (4) allowing bioprinting inside an interior volume thereof is assembled to form a side wall (SW) extending upwardly from a base provided with a target support (20) for the bioprinting. Once an adapter (5) has been coupled to a bioreactor top portion (4b) to plug a corresponding aperture O4, a biological material depositing end (8) may be maintained in an inserted state, extending through and/or being adapted to be fed via the aperture (O4). A sealed enclosure (E), including the side wall and ports, is formed by a sealing operation to interconnect the base and the top portion (4b). Bioprinting is done by driving from outside the depositing end (8), using variation of said volume, for delivering the biological material on the support (20) and then, a culture and/or maturation phase of the printed tissue may start inside the enclosure (E), without displacement of the printed tissue.

公开(公告)号：EP4316842A1

公开(公告)日：2024-02-07

申请号：EP22306180.5

申请日：2022-08-03

申请人： SARTORIUS STEDIM FMT SAS ,  UNIVERSITE CLAUDE BERNARD - LYON 1 ,  Centre National de la Recherche Scientifique (CNRS) ,  Institut National des Sciences Appliquees de Lyon (Insa Lyon) ,  Ecole Superieure de Chimie, Physique, Electronique de Lyon

发明人： Gay, Isabelle ,  Dufour, Alexandre ,  Marquette, Christophe ,  Petiot, Emma ,  Barbaroux, Magali

IPC分类号： B33Y70/00 ,  B29C64/106 ,  C12M1/00 ,  C12M3/00 ,  C12M1/26

摘要： The invention concerns a sterile enclosure (2) intended for a printing of a biological three-dimensional structure, the enclosure comprising:
- a support (4),
- a covering cap (6) comprising a rigid part (22) and a flexible part (24) extending in the prolongation of the rigid part, the covering cap (6) being sealed to the support (4) to delimit an interior chamber (32), at least one portion of the rigid part being a septum (30) adapted to be punctured by a printing needle for printing a biological three-dimensional structure in the interior chamber.
The invention also concerns a biological printing device and a method of printing.

公开(公告)号：EP4316839A1

公开(公告)日：2024-02-07

申请号：EP22306177.1

申请日：2022-08-03

申请人： SARTORIUS STEDIM FMT SAS ,  UNIVERSITE CLAUDE BERNARD - LYON 1 ,  Institut National des Sciences Appliquees de Lyon (Insa Lyon) ,  Ecole Superieure de Chimie, Physique, Electronique de Lyon ,  Centre National de la Recherche Scientifique (CNRS)

发明人： Gay, Isabelle ,  Dufour, Alexandre ,  Marquette, Christophe ,  Petiot, Emma ,  Barbaroux, Magali

IPC分类号： B33Y70/00 ,  B29C64/106 ,  B29C64/209 ,  C12M1/00 ,  C12M3/00 ,  C12M1/26

摘要： The invention concerns a sterile enclosure (91) comprising a container (92) and a printing needle holder device (58) fixed to the container. The container comprising a base wall (92), a side wall (94), the side wall and the base wall delimiting an interior chamber, one inlet port and outlet port and an aperture (24). The printing needle holder device (58) comprising a needle adapter, a printing needle and a flexible sheath having a first end edge fixed to the needle adapter and a second end edge fixed to the aperture of the container, a three dimensional biological structure being intended to be printed on the base wall (92), the interior chamber being intended to receive a cell culture media.

公开(公告)号：EP3650529A1

公开(公告)日：2020-05-13

申请号：EP18290131.4

申请日：2018-11-09

申请人： Sartorius Stedim FMT SAS

发明人： Bendall, Richard ,  Pullin, Jeremy ,  Gay, Isabelle

IPC分类号： C12M1/00

摘要： A method for producing a single-use container, particularly a single-use bioreactor, an assembly system, and a single-use container particularly a single-use bag such as a single-use bioreactor, comprising: a base material 2; and at least one functional element 3 being at least partially connected with the base material 2, wherein the functional element 3 is obtained by a three dimensional printing technique from at least one printable material 5, wherein the printable material 5 is heated during the three dimensional printing thereby at least partly connecting the printed functional element 3 to the base material 2.