公开(公告)号：EP2220218A4

公开(公告)日：2010-12-08

申请号：EP08844000

申请日：2008-10-30

申请人： SCRIPPS RESEARCH INST

发明人： BRUSTAD ERIC ,  BUSHEY MARK L ,  SCHULTZ PETER G

IPC分类号： C12N9/00 ,  C07K17/06 ,  C12P13/22 ,  C12P21/02

CPC分类号： C12P13/222 ,  C07K17/06 ,  C12N9/93 ,  C12N15/67 ,  C12P21/02

公开(公告)号：EP1797177A4

公开(公告)日：2008-07-09

申请号：EP05815319

申请日：2005-09-21

申请人： SCRIPPS RESEARCH INST

发明人： DEITERS ALEXANDER ,  WU NING ,  SCHULZ PETER G ,  KING DAVID ,  CROPP T ASHTON ,  BOSE MOHUA ,  GROFF DAN ,  XIE JIANMING ,  BRUSTAD ERIC

IPC分类号： C12N9/10 ,  C12P21/00

CPC分类号： C12N9/93

摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with a photoregulated amino acid, Ome-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine using these orthogonal pairs.