公开(公告)号：EP0840895B1

公开(公告)日：2006-04-05

申请号：EP96923333.7

申请日：1996-06-19

申请人： SEREX, INC.

发明人： FITZPATRICK, Judith ,  LENDA, Regina

IPC分类号： G01N33/535 ,  G01N33/536 ,  G01N33/58 ,  G01N33/72 ,  G01N33/94

CPC分类号： G01N33/721 ,  G01N33/536 ,  G01N33/58 ,  G01N33/94 ,  Y10S435/975 ,  Y10S436/808

摘要： Receptor:release ligand complexes and kits based thereon are used in a method for assaying for the presence of analyte in a sample, by detecting a released component of the complex -- receptor or ligand. The complexes are stable in the absence of analyte, but unstable when contacted with the analyte, leading to release of the ligand. The term 'release ligand' or 'reland' is applied to such a ligand. Either the released ligand or released receptor is detected by means of a detection system carried by either. The receptor:ligand complex may be formed in situ or may be pre-formed.

公开(公告)号：EP0600936B1

公开(公告)日：1999-10-06

申请号：EP92916797.1

申请日：1992-07-29

申请人： SEREX, INC.

发明人： FITZPATRICK, Judith ,  LENDA, Regina

IPC分类号： G01N33/53 ,  G01N33/58

CPC分类号： G01N33/58 ,  G01N33/53 ,  G01N33/721 ,  G01N33/94 ,  Y10S435/975 ,  Y10S436/815

公开(公告)号：EP0956504A2

公开(公告)日：1999-11-17

申请号：EP98932871.0

申请日：1998-06-26

申请人： SEREX, INC.

发明人： FITZPATRICK, Judith ,  LENDA, Regina ,  ZENG, Zijian

IPC分类号： C07K16 ,  G01N33

CPC分类号： C07K16/18 ,  G01N33/5308 ,  G01N33/68 ,  Y10S436/815 ,  Y10S530/809

摘要： An monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, MD under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.

公开(公告)号：EP1015028A2

公开(公告)日：2000-07-05

申请号：EP98948385.4

申请日：1998-09-18

申请人： SEREX, INC.

发明人： FITZPATRICK, Judith ,  LENDA, Regina

IPC分类号： A61K39/385 ,  A61K39/00

CPC分类号： C07K16/16 ,  A61K39/385 ,  A61K47/646 ,  A61K2039/6081 ,  A61K2039/627 ,  C07K16/18

摘要： A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. High affinity antibodies to the hapten cotinine have been produced using this method. Antibodies to a glycated protein have also been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule. This method has been used to produce an antibody to the glycated protein HbA1c.

公开(公告)号：EP0840895A1

公开(公告)日：1998-05-13

申请号：EP96923333.0

申请日：1996-06-19

申请人： SEREX INC.

发明人： FITZPATRICK, Judith ,  LENDA, Regina

IPC分类号： G01N33

CPC分类号： G01N33/721 ,  G01N33/536 ,  G01N33/58 ,  G01N33/94 ,  Y10S435/975 ,  Y10S436/808

摘要： Receptor:release ligand complexes and kits based thereon are used in a method for assaying for the presence of analyte in a sample, by detecting a released component of the complex -- receptor or ligand. The complexes are stable in the absence of analyte, but unstable when contacted with the analyte, leading to release of the ligand. The term 'release ligand' or 'reland' is applied to such a ligand. Either the released ligand or released receptor is detected by means of a detection system carried by either. The receptor:ligand complex may be formed in situ or may be pre-formed.

公开(公告)号：EP0600936A1

公开(公告)日：1994-06-15

申请号：EP92916797.0

申请日：1992-07-29

申请人： SEREX INC.

发明人： FITZPATRICK, Judith ,  LENDA, Regina

IPC分类号： G01N33

CPC分类号： G01N33/58 ,  G01N33/53 ,  G01N33/721 ,  G01N33/94 ,  Y10S435/975 ,  Y10S436/815

摘要： Un procédé de détermination de la présence d'un analyte dans un échantillon, basé sur l'affinité de liaison différentielle, consiste à détecter la dissociation d'un complexe récepteur-ligand en présence d'un analyte. Le récepteur lie l'analyte avec une affinité élevée et se lie au ligand avec une faible affinité. Le complexe récepteur-ligand peut être formé in situ ou peut être formé préalablement. En présence de l'analyte libre, le récepteur se libère du complexe récepteur-ligand et se lie à l'analyte libre. La libération du récepteur du complexe récepteur-ligand peut être détectée. Du kit permettant de mettre en oeuvre des analyses de détection basées sur la libération afin de détecter la présence de l'analyte est aussi décrit.