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公开(公告)号：EP0565168A3
公开(公告)日：1994-04-13
申请号：EP93200912.9
申请日：1993-03-30
申请人： SOLVAY ENZYMES, INC.
CPC分类号： C11D3/386 , C11D3/0078 , C12N9/00 , C12N9/54 , Y10S435/816
摘要： A method for the preparation of a purified enzyme from a fermentation broth by separating the enzyme solution, adding an organic compound selected from carboxylic acids, amino acids, esters of these acids or blends of these compounds and collecting the purified enzyme precipitate.
摘要翻译： 通过分离酶溶液从发酵液中制备纯化的酶的方法，加入选自羧酸，氨基酸，这些酸的酯或这些化合物的混合物的有机化合物并收集纯化的酶沉淀物。
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2.发明公开Purified alkaline protease concentrate and method of preparation 失效Gereinigtes碱性蛋白酶和Verfahren zur Herstellung。
公开(公告)号：EP0549048A1
公开(公告)日：1993-06-30
申请号：EP92203938.3
申请日：1992-12-16
申请人： SOLVAY ENZYMES, INC.
CPC分类号： C11D3/0078 , C11D3/386 , C12N9/54 , Y10S435/816
摘要： A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20 °C and a pH between pH 4.0 and pH 6.5 at constant agitation.
摘要翻译： 通过包括将碱性蛋白酶水溶液与氯化钠和碳水化合物水解酶在升高的温度下孵育的方法，通过从包含杂质的超滤液中分离含有无定形形式和结晶形式的碱性蛋白酶的混合物来制备高度纯化的碱性蛋白酶制剂 大于20℃，pH在4.0和pH6.5之间。
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3.发明公开Purified enzyme concentrate and method of preparation 失效Gereinigtes Enzym-Konzentrat und Verfahren zur Herstellung。
公开(公告)号：EP0565168A2
公开(公告)日：1993-10-13
申请号：EP93200912.9
申请日：1993-03-30
申请人： SOLVAY ENZYMES, INC.
CPC分类号： C11D3/386 , C11D3/0078 , C12N9/00 , C12N9/54 , Y10S435/816
摘要： A method for the preparation of a purified enzyme from a fermentation broth by separating the enzyme solution, adding an organic compound selected from carboxylic acids, amino acids, esters of these acids or blends of these compounds and collecting the purified enzyme precipitate.
摘要翻译： 本发明涉及从发酵液中制备纯化酶的方法。 本发明还提供含有该纯化酶的组合物。
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