公开(公告)号：EP0325045B1

公开(公告)日：1995-02-08

申请号：EP88312056.0

申请日：1988-12-20

申请人： SYNTEX (U.S.A.) INC.

发明人： Milburn, Gary Lee ,  Houts, Thomas Michael ,  Rabbie, Judith

IPC分类号： G01N33/543 ,  G01N33/569

CPC分类号： G01N33/54306 ,  G01N33/56911 ,  G01N33/571 ,  Y10S435/871 ,  Y10S436/824 ,  Y10S436/828

公开(公告)号：EP0325045A3

公开(公告)日：1990-05-30

申请号：EP88312056.0

申请日：1988-12-20

申请人： SYNTEX (U.S.A.) INC.

发明人： Milburn, Gary Lee ,  Houts, Thomas Michael ,  Rabbie, Judith

IPC分类号： G01N33/543 ,  G01N33/569

CPC分类号： G01N33/54306 ,  G01N33/56911 ,  G01N33/571 ,  Y10S435/871 ,  Y10S436/824 ,  Y10S436/828

摘要： Methods are disclosed for detecting an antigen in a biological sample. The methods involve providing in combination a solid support, which is substantially free of specific binding proteins, and a medium comprising an antigen from the sample and an antibody for the antigen. The combination is incubated under conditions sufficient for the antibody when bound to the antigen to bind to the support. The presence or amount of antibody on the support or in the medium is detemined and is related to the presence of antigen in the sample. The methods have particular application to the detection of gram-negative bacteria.

公开(公告)号：EP0325045A2

公开(公告)日：1989-07-26

申请号：EP88312056.0

申请日：1988-12-20

申请人： SYNTEX (U.S.A.) INC.

发明人： Milburn, Gary Lee ,  Houts, Thomas Michael ,  Rabbie, Judith

IPC分类号： G01N33/543 ,  G01N33/569

CPC分类号： G01N33/54306 ,  G01N33/56911 ,  G01N33/571 ,  Y10S435/871 ,  Y10S436/824 ,  Y10S436/828

摘要： Methods are disclosed for detecting an antigen in a biological sample. The methods involve providing in combination a solid support, which is substantially free of specific binding proteins, and a medium comprising an antigen from the sample and an antibody for the antigen. The combination is incubated under conditions sufficient for the antibody when bound to the antigen to bind to the support. The presence or amount of antibody on the support or in the medium is detemined and is related to the presence of antigen in the sample. The methods have particular application to the detection of gram-negative bacteria.

摘要翻译： 公开了用于检测生物样品中的抗原的方法。 所述方法包括组合提供基本上不含特异性结合蛋白的固体支持物和包含来自样品的抗原和抗原抗体的培养基。 当结合抗原结合载体时，将该组合在足以使抗体条件下培养。 确定载体或介质中抗体的存在或量，并与样品中抗原的存在有关。 该方法特别适用于革兰氏阴性菌的检测。