公开(公告)号：EP2089506A2

公开(公告)日：2009-08-19

申请号：EP07819624.3

申请日：2007-11-06

申请人： Saxonia BioTec GmbH

发明人： LUNING, Rudolph ,  KLAUS, Uwe ,  PURCELL, Laurence J. ,  DOERING, Stefan

IPC分类号： C12M1/12 ,  C12M1/20 ,  C12M3/04

CPC分类号： C12M23/44 ,  C12M23/34 ,  C12M29/02 ,  C12M41/00 ,  F04B43/028 ,  F04B43/043

摘要： A device for supplying cell culture modules with nutrients has an arrangement of channels, pumps and valves in or on a plate. The valves may be pinch valves operated by deforming an elastic cover over a solid body and the pump may be a pinch valve pump. The channels may be defined, at least in part, by the plate. The pumps, channels and valves may be located within the thickness of the plate and the cover. The device may be used to supply nutrients to cell culture modules according to a perfusion operation, a re-circulation operation and/or a combination of both. A pump may comprise a generally rigid solid body and a seal. The solid body may wholly or partially define an inlet channel, a plenum and an outlet channel. A port between the inlet channel and the plenum is covered by the seal. A surface of the plenum is deformable. Deforming the surface forces liquid in the plenum to push the seal to cover the inlet channel port and to flow through the outlet channel. When the surface is returned to its original position, fluid flows into the plenum at least partially through the inlet channel displacing or deforming the seal so as to allow flow through the port.