公开(公告)号：EP0195773B1

公开(公告)日：1989-11-08

申请号：EP84903493.9

申请日：1984-09-19

申请人： Secretary of State for Defence in Her Britannic Majesty's Gov. of the United Kingdom of Great Britain and Northern Ireland

发明人： ELLWOOD, Derek, Clifford ,  EVANS, Charles, Gervase, Thorngate ,  YEO, Richard, Grenville

IPC分类号： C12P19/06

CPC分类号： C12P19/06

摘要： Polysaccharide is produced by a single stage continuous culture of Xanthomonas bacteria, especially of the Xanthomonas campestris group, in a chemically defined culture medium having a growth limiting nutrilite which is a nitrogen source selected from glutamic acid, asparagine, a glutamate salt or an asparagine salt. During the continuous process the culture has a mean residence time in the culture vessel of 16 hr 2/3 or less, preferably between 9 and 16 hr 2/3. The use of glutamic acid, asparagine, glutamate salt or asparagine salt as the limiting nitrogen nutrilite increases the output of polysaccharide at mean residence times of 16 hr 2/3 and below, when compared with the outputs achieved using inorganic nitrogen sources at the same mean residence times.