公开(公告)号：EP2075327A1

公开(公告)日：2009-07-01

申请号：EP07721549.9

申请日：2007-06-25

申请人： Shanghai Apple Flavor&Fragrance Co. Ltd. ,  Shanghai Kaixin Biotech Co., Ltd

发明人： XU, Ping ,  HUA, Dongliang ,  LIN, Shan ,  SONG, Lifu ,  MA, Cuiqing ,  ZHANG, Zhaobin ,  ZENG, Yiyong ,  DU, Yi ,  CHEN, Hong ,  GAN, Li ,  WEI, Zhonghao

IPC分类号： C12N1/04 ,  C12N1/20 ,  C12P7/24 ,  C12R1/01 ,  C12R1/465

CPC分类号： C12R1/465 ,  C12P7/24

摘要： A method of vanillin production from ferulic acid with a high concentration by biotransformation using a Streptomyces sp. strain is claimed in this invention. This strain is named as Streptomyces sp. V-1, which has been deposited in China Center for Type Culture Collection on July 12, 2006 with the number of CCTCC M 206065. Using this strain, high concentration of vanillin fermentation broth is obtained from ferulic acid by biotransformation in GY biotransformation medium. With the addition of macroporous adsorbent resin DM11, the concentration of vanillin can be greatly improved. The advantage of this invention is less environmental pollution, high product concentration, less by-product, short processing cycle, low production cost, simple product extraction, clean production process, product environmental friendly, safe and reliable, which solves many difficulties in the vanillin production from botanical raw material extraction or chemical synthesis, and therefore it has good application prospect.

摘要翻译： 香兰素从生产阿魏酸的高浓度通过使用链霉菌属生物转化的方法。 应变是本发明要求保护的。 这株被命名为链霉菌。 V-1，已经沉积在中国中心典型培养物保藏于2006年7月12日，与CCTCC中号第二十零万六千零六十五数量使用该菌株，香兰素发酵液中的高浓度由GY转化培养基中的生物转化从阿魏酸获得。 通过添加大孔吸附树脂DM11的，香草醛的浓度可以大大提高。 本发明的优点是环境污染少，产品的高浓度，更少的副产物，加工周期短，生产成本低，简单的产品提取，清洁生产工艺，产品环保，安全可靠，这在香兰素解决了许多困难 生产从植物原料提取或化学合成的，因此它具有良好的应用前景。