公开(公告)号：EP2447718A1

公开(公告)日：2012-05-02

申请号：EP11187319.6

申请日：2006-12-19

申请人： Sharp Kabushiki Kaisha

发明人： Reeve, Michael A. ,  Anderson, Sally

IPC分类号： G01N33/531 ,  C07K7/06 ,  C07K7/08 ,  G01N33/543

CPC分类号： C07K7/08 ,  C07K7/06 ,  G01N33/531 ,  G01N33/54386 ,  G01N33/6845

摘要： An array of peptide dimer ligand-binding capture agents for capturing an analyte in a sample wherein each of said ligand-binding capture agents comprises first and second peptide chains being formed as a dimer by means of a covalent link therebetween, wherein said covalent link has been formed by reaction of first and second thiol groups on said first and second peptides, each of said first and second peptide chains comprises a chain of between 5 and 25 amino acids wherein each amino acid is a D-enantiomer or an L-enantiomer, each of said first and second peptide chains have different amino acid sequences, said first peptide is immobilised via an attachment moiety to a substrate, said attachment moiety being selected from the group consisting of a hydrophobic moiety and a moiety forming a covalent bond with the substrate and wherein the first and second peptide chains have amino acid sequences which are combinatorially-varied and the array comprises discrete spatially addressable locations and each location comprises a different capture agent.

摘要翻译： 用于捕获样品中分析物的肽二聚体配体结合捕获剂的阵列，其中每个所述配体结合捕获剂包含通过其间的共价连接形成为二聚体的第一和第二肽链，其中所述共价连接具有 通过所述第一和第二肽上的第一和第二硫醇基团的反应形成，所述第一和第二肽链中的每一个包含5至25个氨基酸的链，其中每个氨基酸是D-对映异构体或L-对映异构体， 所述第一和第二肽链中的每一个具有不同的氨基酸序列，所述第一肽通过附着部分固定至底物，所述连接部分选自疏水部分和与底物形成共价键的部分 并且其中所述第一和第二肽链具有组合变化的氨基酸序列，并且所述阵列包括离散的空间可寻址定位 离子和每个位置包括不同的捕获剂。