公开(公告)号：EP4394031A1

公开(公告)日：2024-07-03

申请号：EP23856254.0

申请日：2023-06-27

申请人： Shenzhen Beike Biotechnology Co., Ltd.

发明人： LIAO, Yan ,  FU, Zeqin ,  LI, Duanduan ,  YANG, Yulin ,  HUANG, Yinfu ,  CAI, Cheguo ,  HU, Junyuan

IPC分类号： C12N5/0775 ,  A61K35/28 ,  A61P37/02 ,  A61P29/00

摘要： The present invention discloses a mesenchymal stem cell treated by at least two of cytokines IL4, IL21, and IL27, an exosome and use thereof. The present invention relates to the technical field of biomedicine; the mesenchymal stem cell and exosome thereof are obtained after the mesenchymal stem cell is pretreated with a cytokine composition. The mesenchymal stem cell is pretreated in a way of: treating and stimulating the mesenchymal stem cell by stages with a complete medium containing the cytokine composition to obtain a capacitated mesenchymal stem cell; the exosome of the mesenchymal stem cell is pretreated in a way of: treating and stimulating the mesenchymal stem cell by stages with a complete medium containing the cytokine composition, and replacing with a basal medium for starvation treatment. The mesenchymal stem cell obtained after being pretreated with the cytokine composition and an exosome thereof provided in the present invention can exert a stronger immune modulating effect and thus, can be used for the treatment of immune diseases better.