公开(公告)号：EP3950927A1

公开(公告)日：2022-02-09

申请号：EP20781770.1

申请日：2020-04-01

申请人： Spiber Inc.

发明人： KOBAYASHI Masato ,  KURACHI Kenji ,  MORI Eiji ,  NODA Takanobu

IPC分类号： C12N1/15 ,  C12P21/02

摘要： The present invention relates to a method for producing a recombinant protein, the method including: a first step of culturing a recombinant cell expressing a recombinant protein under control of an inducible promoter in a medium containing galactose and at least two selected from the group consisting of mannitol, raffinose, and melibiose; and a second step of further culturing the recombinant cell under a condition that expression from the inducible promoter is induced after the first step.