公开(公告)号：EP1893975A2

公开(公告)日：2008-03-05

申请号：EP06784804.4

申请日：2006-06-12

申请人： Stratagene California

发明人： REID, Taylor, A. ,  TAYLOR, Roger, H. ,  ZAJAC, Kenneth, J.

IPC分类号： G01N21/01

CPC分类号： G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

摘要： A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tube (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filter (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).

公开(公告)号：EP1943655A2

公开(公告)日：2008-07-16

申请号：EP06813856.9

申请日：2006-08-25

申请人： Stratagene California

发明人： REID, Taylor, A. ,  TAYLOR, Roger, H.

IPC分类号： G21H3/02

CPC分类号： G01N21/6452 ,  G01N21/6428 ,  G01N2201/062 ,  G01N2201/0642 ,  G01N2201/101 ,  H01L31/167 ,  H01L2224/48472 ,  H01L2924/10253 ,  H01L2924/3025 ,  H01L2224/48091 ,  H01L2924/00014 ,  H01L2924/00

摘要： A compact optical module for fluorescence excitation and detection and methods for using same are disclosed. An apparatus for detecting fluorescence includes a substrate base, a detector adjacent to the substrate base for determining the amount of fluorescence, an emission filter adjacent to the detector, a light source for emitting an excitation light, the light source engaging the emission filter, and a cover formed over the detector, the emission filter, and the light source.

公开(公告)号：EP1880175A2

公开(公告)日：2008-01-23

申请号：EP06758923.4

申请日：2006-05-02

申请人： Stratagene California

发明人： TAYLOR, Roger, H. ,  REID, Taylor, A. ,  CHOI, Howard, Y.

IPC分类号： G01J3/30 ,  G01N21/64

CPC分类号： G01J3/10 ,  G01J3/4406 ,  G01J2001/4242 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486

摘要： A system and method for a pulsed light source (40) used in detecting fluorescence from a plurality of samples (94) of biological material discretely, continuously or intermittently during thermal cycling of DNA to accomplish a polymerase chain reaction (PCR). An apparatus for sampling at least one sample (94) of a biological material comprises a light source (40) that emits a pulsed excitation light (42) that interacts with the sample (94) and a detector (50) sensitive to fluorescence emitted from the sample (94). A method of sampling at least one sample (94) to detect fluorescence comprises generating a pulsed excitation light (42) with a pulsed light source (40); directing the pulsed excitation light (42) into the sample (94); illuminating a sample (94) with the pulsed excitation light (42) to generate an emission light; and detecting the optical characteristics of the emission light.