公开(公告)号：EP0882983B1

公开(公告)日：2005-04-06

申请号：EP98109089.7

申请日：1998-05-19

申请人： Sysmex Corporation

发明人： Mizukami, Toshihiro ,  Ishyama, Munetaka ,  Sakata, Takashi

IPC分类号： G01N33/50 ,  G01N1/30 ,  G01N33/52 ,  G01N33/80 ,  C09B23/02

CPC分类号： G01N33/52 ,  G01N33/5094 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

公开(公告)号：EP0882983A3

公开(公告)日：2001-02-07

申请号：EP98109089.7

申请日：1998-05-19

申请人： Sysmex Corporation

发明人： Mizukami, Toshihiro ,  Ishyama, Munetaka ,  Sakata, Takashi

IPC分类号： G01N33/50 ,  G01N1/30 ,  G01N33/52 ,  G01N33/80 ,  C09B23/02

CPC分类号： G01N33/52 ,  G01N33/5094 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

摘要： A reagent for classification and counting of leukocytes, the reagent containing at least one dye which has the following structural formula    where R 1 is a hydrogen atom or an alkyl group, R 2 and R 3 each represent a hydrogen atom, a lower alkyl group or a lower alkoxy group, R 4 represents a hydrogen atom, an acyl group or an alkyl group, Z represents a sulfur atom, an oxygen atom, or a carbon atom having a lower alkyl group, n denotes 0, 1 or 2, and X - represents an anion, and specifically binds to RNA to increase in fluorescence intensity, and a method for classification and counting of leukocytes which uses the reagent, whereby abnormal cells such as immature leukocytes and abnormal leukocytes can be classified and counted easily and highly accurately, and at the same time, classification and counting of normal leukocytes as well as the counting of leukocytes can be performed.