公开(公告)号：EP1225229B1

公开(公告)日：2011-10-19

申请号：EP00969984.4

申请日：2000-10-23

申请人： TAKARA BIO INC.

发明人： UENO, Takashi ,  MATSUMURA, Hajime ,  TANAKA, Keiji ,  IWASAKI, Tomoko ,  UENO, Mitsuhiro ,  FUJINAGA, Kei ,  ASADA, Kiyozo ,  KATO, Ikunoshin

IPC分类号： C12N15/861 ,  A61K35/12 ,  A61K35/76 ,  A61K48/00 ,  C12N5/10 ,  C12N7/01

CPC分类号： C12N15/86 ,  C12N2710/10343 ,  C12N2800/30

摘要： A method of transferring a foreign gene into cells, characterized by involving: the step of transferring into the cells with the use of an adenovirus vector, a first nucleic acid, which has a sequence provided with adeno-associated virus-origin ITRs in both sides of the target foreign gene to be transferred, and a second nucleic acid, which has an adeno-associated virus-origin rep gene and a promoter for expressing this gene and carries a stuffer sequence inserted thereinto sandwiched in two recombinase recognition sequences and located between the rep gene and the promoter; and the step of expressing the Rep protein under the action of recombinase in the cells obtained in the above step to thereby integrate the target foreign gene into the chromosomal DNA.

公开(公告)号：EP1319713B1

公开(公告)日：2010-01-27

申请号：EP01965692.5

申请日：2001-09-18

申请人： TAKARA BIO INC.

发明人： CHONO, Hideto ,  MATSUMURA, Hajime ,  UENO, Mitsuhiro ,  KATO, Ikunoshin

IPC分类号： C12N15/48 ,  C12N15/867 ,  C07K14/15

CPC分类号： C12N15/907 ,  A01K2217/05 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N15/90 ,  C12N2740/13022 ,  C12N2740/13043

摘要： A complex comprising a double-stranded DNA containing a terminal repeated sequence originating in a retrovirus and a DNA having a base sequence which does not occur in this retrovirus and a retrovirus-origin protein component.

公开(公告)号：EP2138580A1

公开(公告)日：2009-12-30

申请号：EP08740493.5

申请日：2008-04-16

申请人： Takara Bio, Inc.

发明人： CHONO, Hideto ,  MATSUMOTO, Kazuya ,  MATSUMURA, Hajime ,  MINENO, Junichi ,  KATO, Ikunoshin

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K38/46 ,  A61K48/00 ,  A61P43/00 ,  C12N5/10

CPC分类号： C12N9/22 ,  A61K38/00 ,  A61K48/00 ,  A61K48/005 ,  C12N15/86 ,  C12N2740/16043

摘要： The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections.

摘要翻译： 本发明提供了含有转录单位的逆转录病毒载体，其包含转录调控序列和编码具有单链RNA特异性核糖核酸内切酶活性的多肽的基因，其被置于其表达可以通过调节序列控制， 使得来自该单位的mRNA的转录方向与逆转录病毒载体的RNA基因组的转录方向相反。 通过使用如上所述构建的载体，可以制备显示高基因转移效率的病毒上清液。 本发明的逆转录病毒载体可用于治疗和/或预防癌症和病毒感染。

公开(公告)号：EP2138580B1

公开(公告)日：2013-03-20

申请号：EP08740493.5

申请日：2008-04-16

申请人： Takara Bio, Inc.

发明人： CHONO, Hideto ,  MATSUMOTO, Kazuya ,  MATSUMURA, Hajime ,  MINENO, Junichi ,  KATO, Ikunoshin

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K38/46 ,  A61K48/00 ,  A61P43/00 ,  C12N5/10 ,  C12N9/22

CPC分类号： C12N9/22 ,  A61K38/00 ,  A61K48/00 ,  A61K48/005 ,  C12N15/86 ,  C12N2740/16043

公开(公告)号：EP1319713A1

公开(公告)日：2003-06-18

申请号：EP01965692.5

申请日：2001-09-18

申请人： TAKARA BIO INC.

发明人： CHONO, Hideto ,  MATSUMURA, Hajime ,  UENO, Mitsuhiro ,  KATO, Ikunoshin

IPC分类号： C12N15/48 ,  C12N15/867 ,  C07K14/15

CPC分类号： C12N15/907 ,  A01K2217/05 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N15/90 ,  C12N2740/13022 ,  C12N2740/13043

摘要： A complex comprising a double-stranded DNA containing a terminal repeated sequence originating in a retrovirus and a DNA having a base sequence which does not occur in this retrovirus and a retrovirus-origin protein component.

摘要翻译： 包含含有源自逆转录病毒的末端重复序列的双链DNA和具有不存在于该逆转录病毒中的碱基序列的DNA和逆转录病毒起源蛋白质成分的复合体。