专利检索 ap:("THE JOHNS HOPKINS UNIVERSITY") AND inv:"JOHNSON, Karen A." 第 1 页

1.

发明公开
ANTIBODIES AND ASSAYS FOR DETERMINING MUTATIONS IN THE $i(APC) GENE 失效
标题翻译：抗体和方法在APC基因突变厘定

公开(公告)号：EP0689606A1

公开(公告)日：1996-01-03

申请号：EP94911656.0

申请日：1994-03-21

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： HILL, David E. , JOHNSON, Karen A. , KINZLER, Kenneth W. , VOGELSTEIN, Bert

IPC分类号： G01N33 , C07K14 , C07K16 , C12N5 , C12N15 , C12P21 , C12R1

CPC分类号： C07K16/18 , C07K14/47 , C07K16/22 , C07K2319/00

摘要： Antibodies and assays employing them are taught which are useful for detecting the bulk of mutations which occur in the APC gene in familial adenomatous polyposis. The antibodies are specific for epitopes in the amino terminal or carboxy terminal portion of the protein. A variety of immunoassay formats are described which are all based on the observation that the bulk of the APC mutations which occur in familial adenomatous polyposis and sporadic colorectal carcinomas result in truncated APC proteins. Generally, either the size of APC proteins is determined or the relative binding of amino terminal-binding antibodies to carboxy terminal-binding antibodies is determined.