公开(公告)号：EP1328656A1

公开(公告)日：2003-07-23

申请号：EP01977386.0

申请日：2001-10-01

申请人： The Johns Hopkins UNiversity School of Medicine

发明人： BAYLIN, Stephen, B. ,  HERMAN, James, G. ,  ESTELLER, Manel

IPC分类号： C12Q1/48 ,  C12Q1/37 ,  A61K38/51

CPC分类号： C07K16/40 ,  C12Q1/683 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/154 ,  Y10S435/975 ,  C12Q2523/125

摘要： The present invention provides methods relating to chemotherapeutic treatment of a cell proliferative disorder. In particular, a method is provided for predicting the clinical response to certain types of chemotherapeutic agents. Aklylating agents, used for the treatment of certain types of tumors including tumors of the nervous system and lymph system, are efficacious agents when the damage they do to tumor cell DNA is not repaired by cellular DNA repair mechanisms. The present invention provides a method for determining the activity of a gene encoding a DNA repair enzyme, thus providing a prediction of the clinical response to alkylating agents. Figure (1) shows the overall survival among subjects with gliomas treated carmustine, and the time of progression of disease according to the methylation status of the MGMT promoter.

公开(公告)号：EP0954608A1

公开(公告)日：1999-11-10

申请号：EP97927933.0

申请日：1997-06-03

申请人： THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE

发明人： HERMAN, James, G. ,  BAYLIN, Stephen, B.

IPC分类号： C12N15 ,  C12Q1

CPC分类号： C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2523/125 ,  C12Q2531/113 ,  C12Q2525/185 ,  C12Q2535/125 ,  C12Q2521/331

摘要： The present invention provides a method of PCR, methylation specific PCR (MSP), for rapid identification of DNA methylation patterns in a CpG-containing nucleic acid. MSP uses the PCR reaction itself to distinguish between methylated and unmethylated DNA, which adds an improved sensitivity of methylation detection.

公开(公告)号：EP0954608B1

公开(公告)日：2006-05-17

申请号：EP97927933.8

申请日：1997-06-03

申请人： THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE

发明人： HERMAN, James, G. ,  BAYLIN, Stephen, B.

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2523/125 ,  C12Q2531/113 ,  C12Q2525/185 ,  C12Q2535/125 ,  C12Q2521/331

摘要： The present invention provides a method of PCR, methylation specific PCR (MSP), for rapid identification of DNA methylation patterns in a CpG-containing nucleic acid. MSP uses the PCR reaction itself to distinguish between methylated and unmethylated DNA, which adds an improved sensitivity of methylation detection.