公开(公告)号：EP0505564B1

公开(公告)日：1997-01-02

申请号：EP92900681.5

申请日：1991-10-23

申请人： THE PERKIN-ELMER CORPORATION

发明人： DOLLINGER, Gavin, D. ,  CUNICO, Robert, L. ,  KUNITANI, Michael, G.

IPC分类号： G01N15/02 ,  G01N30/74

CPC分类号： G01N15/0205 ,  G01N15/04 ,  G01N21/532 ,  G01N30/74 ,  G01N33/68 ,  G01N2021/4726 ,  Y10T436/25375

摘要： A high angle light scattering detector using classical Rayleigh scattering. A high intensity arc light source, filtered to leave only one wavelength illuminates a flow cell. Through the flow cell, very small particles such as biological proteins flow in solution after separation by HPLC or some other means. A UV detector generates data regarding the weight concentration of the eluting particles and a scattered light detector collecting scattered light at angles of approximately 90° generates a scattered light signal. The incident light intensity is also measured. The average molecular weight is then computed using the scattered and incident light data, the weight concentration data and a simplified mathematical relationship from which the size factor P and the viral coefficients have been eliminated.