公开(公告)号：EP1296720A2

公开(公告)日：2003-04-02

申请号：EP01928531.1

申请日：2001-04-13

申请人： THE UAB RESEARCH FOUNDATION

发明人： MORROW, Casey, D. ,  JACKSON, Cheryl ,  PEDUZZI, Jean

IPC分类号： A61K48/00 ,  C12N15/86 ,  A61P25/00 ,  A61P35/00 ,  A61P31/04

CPC分类号： A61K39/105 ,  A61K38/191 ,  A61K48/00 ,  A61K2039/5256 ,  C12N15/86 ,  C12N2770/32634 ,  C12N2770/32643 ,  Y02A50/466

摘要： The invention pertains to methods of delivering a polypeptide to a cell comprising (a) contacting a cell with a replicon having a non-poliovirus nucleic acid substituted for a nucleic acid which encodes at least a portion of a protein necessary for encapsidation, the non-poliovirus nucleic acid encoding, in an expressible form, a polypeptide or fragment thereof; and (b) maintaining the cells under conditions appropriate for introduction of the replicons into the cells. The cell may be within a subject and the polypeptide may be a therapeutic agent. The methods of the invention may be used to treat diseases including central nervous system disorders, infectious diseases, and cancer.