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公开(公告)号：EP0856055A1
公开(公告)日：1998-08-05
申请号：EP96916428.0
申请日：1996-04-10
发明人： SANO, Takeshi , CANTOR, Charles, R. , VAJDA, Sandor , REZNIK, Gabriel, O. , SMITH, Cassandra, L. , PANDORI, Mark, W.
CPC分类号： G01N33/538 , A61K38/00 , C07K14/36 , G01N2333/465 , Y10S530/808 , Y10S530/81
摘要： The present invention relates to streptavidin proteins and peptides having altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.
摘要翻译： 本发明涉及链霉抗生物素蛋白和肽，其具有改变的物理性质，例如稳定性提高或对结合生物素的亲和力增加或降低。 本发明还涉及使用链霉抗生物素蛋白或肽检测，鉴定，分离和分离靶标的方法。 具有增加的或降低的亲和力的链霉抗生物素蛋白允许使用链霉抗生物素蛋白 - 生物素偶联系统用于检测和分离系统，其中有必要除去一个或另一个结合伴侣。 这样的系统可用于功能性蛋白质和活细胞的纯化。 本发明还涉及编码这些链霉抗生物素蛋白和肽的核酸，以及包含这些核酸的重组细胞，例如细菌，酵母和哺乳动物细胞。
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公开(公告)号：EP0977770A1
公开(公告)日：2000-02-09
申请号：EP98909164.0
申请日：1998-03-13
CPC分类号： C07K14/36
摘要： Compounds and methods are described for producing streptavidin mutants with changed affinities. In particular, modifications to the sequence of the natural streptavidin gene is described to create amino acid substitutions resulting in greater affinity for biotin subsitutes than for biotin.
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