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    METHODS FOR DETERMINING MULTIPLE INTERACTIONS BETWEEN NUCLEIC ACIDS IN A CELL
    1.
    发明公开
    METHODS FOR DETERMINING MULTIPLE INTERACTIONS BETWEEN NUCLEIC ACIDS IN A CELL 审中-公开
    用于确定NUCLEIC之间的多重相互作用在细胞

    公开(公告)号：EP2971278A1

    公开(公告)日：2016-01-20

    申请号：EP14764060.1

    申请日：2014-03-14

    申请人： The Broad Institute, Inc. ,  President and Fellows of Harvard College

    发明人： RAO, Suhas ,  STAMENOVA, Elena ,  AIDEN, Erez Lieberman

    IPC分类号： C40B30/00

    CPC分类号： C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q2565/133

    摘要： Disclosed are methods for detecting spatial proximity relationships between nucleic acid sequences in a cell. The methods include: providing a sample of one or more cells comprising nucleic acids; fragmenting the nucleic acids present in the cells, wherein the fragmented nucleic acids have ends capable of joining to other fragmented nucleic acids; joining ends of fragmented nucleic acids to other ends fragmented nucleic acid to create at least one nucleic acid concatemer having at least one junction between the joined fragmented nucleic acids, and wherein the at least one nucleic acid concatemer encodes the information about the proximity of the DNA sequences in the cell; and determining the sequence at least one junction of the at least one nucleic acid concatemer, thereby detecting spatial proximity relationships between nucleic acid sequences in a cell.