公开(公告)号：EP2994754A1

公开(公告)日：2016-03-16

申请号：EP14729563.8

申请日：2014-05-12

申请人： The Government of The United States of America as represented by The Secretary, Department of Health and Human Services

发明人： KHUDYAKOV, Yury, E. ,  OBRIADINA, Anna

IPC分类号： G01N33/50

CPC分类号： G01N33/5767 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/24222 ,  C12N2770/24231 ,  G01N2333/186 ,  G01N2469/20

摘要： Compositions and processes are provided for the robust detection of hepatitis C virus in a sample. Compositions include amino acid sequences of HCV core region including or exclusive to residues 14-31 or 50-90 of the HCV core protein. Also provided are processes of detecting HCV in a sample whereby the provided peptides are optionally used alone or in conjunction with antibodies that do not recognize the peptides so that detection is independent of seroconversion in a subject. Using the compositions and processes, HCV can be detected in a sample prior to or following seroconversion leading to robust HCV detection and diagnosis.

摘要翻译： 提供了组合物和方法，用于在样品中强力检测丙型肝炎病毒。 组合物包括HCV核心区域的氨基酸序列，包括或排除HCV核心蛋白质残基14-31或50-90的氨基酸序列。 还提供了在样品中检测HCV的方法，其中所提供的肽任选地单独使用或与不识别肽的抗体结合，使得检测不依赖于受试者的血清转化。 使用组合物和方法，可以在血清转化之前或之后在样品中检测HCV，从而导致强烈的HCV检测和诊断。

公开(公告)号：EP1200465A1

公开(公告)日：2002-05-02

申请号：EP00948602.8

申请日：2000-07-07

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by The Secretary, Department of Health and Human Services

发明人： FIELDS, Howard, A. ,  KHUDYAKOV, Yury, E.

IPC分类号： C07K14/18 ,  A61K39/29

CPC分类号： C07K14/005 ,  A61K2039/525 ,  C12N2770/24222

摘要： Antigenic epitopes of hepatitis C virus (HCV) and mosaic HCV polypeptides useful as reagents in assays for the diagnosis or monitoring of HCV in a biological sample. The antigenic epitopes and mosaic polypeptides are also useful for the construction of immunogenic pharmaceutical compositions, such as vaccines. The mosaic polypeptides are artificial composite proteins constructed from diagnostically relevant antigenic regions derived from different HCV proteins. Preferably, the mosaic polypeptides contain antigenic epitopes from the core protein, NS3 protein, and NS4 protein. The preferred mosaic polypeptides optionally contain an additional antigenic epitope from either the NS4 protein or the NS5a protein or both.

公开(公告)号：EP1274725B1

公开(公告)日：2006-06-07

申请号：EP01924610.7

申请日：2001-04-03

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services

发明人： FIELDS, Howard, A. ,  KHUDYAKOV, Yury, E. ,  MENG, Jihong

IPC分类号： C07K14/005

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2770/28122

摘要： The present invention provides immunogenic Hepatitis E Virus polypeptides and methods of use. The polypeptides include at least one neutralizing antigenic epitope and preferably contain at least about fifty amino acids residues between amino acid residues 452 and 617 from the C-terminal of the HEV pORF2 protein. The polypeptides are useful, alone or in combination with other polypeptides of the present invention, as a reagent for studying the pathogenesis of HEV and monitoring treatment efficacy in subjects undergoing treatment for HEV. Further, the polypeptides can be used as a vaccine to treat or prevent HEV.

公开(公告)号：EP1012312B1

公开(公告)日：2007-01-24

申请号：EP98944483.1

申请日：1998-08-21

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： KHUDYAKOV, Yury, E. ,  FIELDS, Howard, A.

IPC分类号： C12N15/62 ,  C07K19/00 ,  C07K14/18 ,  G01N33/576

CPC分类号： C07K14/005 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C12N15/62 ,  C12N2770/24222 ,  Y10S530/826

摘要： A mosaic protein comprising a variety of immunoreactive antigenic epitopes from several genotypes of hepatitis C virus. The mosaic protein provides a sensitive and specific immunological hepatitis detection assay. A restriction enzyme assisted ligation method of making an artificial gene permits controlled construction of mosaic proteins, and allows confirmatory expression of the intermediate gene products.

公开(公告)号：EP1012312A1

公开(公告)日：2000-06-28

申请号：EP98944483.1

申请日：1998-08-21

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by The Secretary, Department of Health and Human Services

发明人： KHUDYAKOV, Yury, E. ,  FIELDS, Howard, A.

IPC分类号： C12N15/62 ,  C07K19/00 ,  C07K14/18 ,  G01N33/576

CPC分类号： C07K14/005 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C12N15/62 ,  C12N2770/24222 ,  Y10S530/826

摘要： A mosaic protein comprising a variety of immunoreactive antigenic epitopes from several genotypes of hepatitis C virus. The mosaic protein provides a sensitive and specific immunological hepatitis detection assay. A restriction enzyme assisted ligation method of making an artificial gene permits controlled construction of mosaic proteins, and allows confirmatory expression of the intermediate gene products.