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    Primers and probes for detection and discrimination of types and subtypes of influenza viruses
    1.
    发明公开
    Primers and probes for detection and discrimination of types and subtypes of influenza viruses 有权
    引物和检测探针和种类分化的流感病毒亚型

    公开(公告)号：EP2360281A2

    公开(公告)日：2011-08-24

    申请号：EP11151493.1

    申请日：2007-02-12

    申请人： The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services

    发明人： Lindstrom, Stephen ,  Klimov, Alexander ,  Cox, Nancy ,  Loftin, Lamorris

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/701 ,  C12Q1/6837

    摘要： Methods of detecting influenza, including differentiating between type and subtype are disclosed, for example to detect, type, and/or subtype an influenza infection. A sample suspected of containing a nucleic acid of an influenza virus, is screened for the presence or absence of that nucleic acid. The presence of the influenza virus nucleic acid indicates the presence of influenza virus. Determining whether the influenza virus nucleic acid is present in the sample can be accomplished by detecting hybridization between an influenza specific probe, influenza type specific probe, and/or subtype specific probe and an influenza nucleic acid. Probes and primers for the detection, typing and/or subtyping of influenza virus are also disclosed. Kits and arrays that contain the disclosed probes and/or primers also are disclosed.

    摘要翻译： 检测流感，包括类型和子类型之间进行区分的方法是游离缺失盘，例如，以检测，类型，和/或流感病毒感染的亚型。 怀疑含有流感病毒的核酸的样品，进行筛选存在或不存在的核酸的那样。 流感病毒核酸的存在指示流感病毒的存在。 确定所述流感病毒核酸是否存在于样品中可以通过检测特定流感型流感特异性探针，和/或亚型特异性探针的探针之间的杂交来实现和甲型流感核酸。 因此探针和引物用于检测，打字和/或流感病毒的亚型是游离缺失盘。 试剂盒和阵列确实含有游离缺失圆盘探针和/或引物，从而被游离缺失光盘。