公开(公告)号：EP1994209A2

公开(公告)日：2008-11-26

申请号：EP07835647.4

申请日：2007-01-03

申请人： The President and Fellows of Harvard College ,  Dana-Farber Cancer Institute

发明人： BARNES, David, W. ,  KOEHLER, Angela, N. ,  BRADNER, James, E. ,  MAZITSCHEK, Ralph ,  SCHREIBER, Stuart, L.

IPC分类号： C40B30/04

CPC分类号： G01N33/6803 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/0074 ,  C40B30/04 ,  C40B40/04 ,  C40B80/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54353 ,  G01N33/547 ,  G01N33/552 ,  G01N33/553

摘要： The present invention provides compositions and methods to facilitate the identification of compounds that are capable of interacting with a biological macromolecule of interest. In one aspect, a composition is provided that comprises an array of one or more types of chemical compounds attached to a solid support using isocyanate or isothiocyanate chemistry, wherein the density of the array of compounds is at least 1000 spots per cm2. In general, these inventive arrays are generated by: (1) providing a solid support, wherein said solid support is functionalized with an isocyanate or isothiocyanate moiety capable of interacting with a desired chemical compound to form a covalent attachment; (2) providing one or more solutions of one or more types of compounds to be attached to the solid support; (3) delivering said one or more types of compounds to the solid support; and (4) catalyzing the attachment of the compound to the solid support, whereby an array is formed and the array of compounds has a density of at least 1000 spots per cm2. In another aspect, the present invention provides methods for utilizing these arrays to identify small molecule partners for biological macromolecules of interest.