公开(公告)号：EP3175302A2

公开(公告)日：2017-06-07

申请号：EP15827025.6

申请日：2015-07-31

申请人： The Regents of the University of California

发明人： OZCAN, Aydogan ,  GREENBAUM, Alon ,  ZHANG, Yibo ,  FEIZI, Alborz ,  LUO, Wei

IPC分类号： G03H1/04 ,  G03H1/00

CPC分类号： G03H1/0005 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1475 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1445 ,  G01N2015/1454 ,  G03H1/0443 ,  G03H1/0866 ,  G03H1/0891 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/0454 ,  G03H2001/046 ,  G03H2001/0816 ,  G03H2001/0883 ,  G03H2001/2655 ,  G03H2210/42 ,  G03H2226/02 ,  G03H2227/03 ,  H04N5/2256 ,  H04N5/23238

摘要： A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

摘要翻译： 一种用于在样品中的样本或对象的无透镜成像方法使用多高度迭代阶段的检索和旋转场变换到具有临床类似的图像质量到基于透镜的台式显微镜进行病理样品的宽FOV成像。 的传输的强度（TIE）方程的解是在相位恢复方法中用作初始猜测在加快恢复过程图像。 所述全息重建图像可以数字聚焦在无需任何聚焦调整对象FOV（残像捕获）内的任何深度，并且因此数字为工件从样品和传感器平面之间不受控制的倾斜和高度变化所产生校正。 在替代实施例中，合成孔径方法用于多角度迭代相位恢复执行病理学样品的宽FOV成像和提高图像的有效数值孔径。