公开(公告)号：EP1216100A1

公开(公告)日：2002-06-26

申请号：EP00964445.1

申请日：2000-09-29

申请人： The Secretary of State For Defence in Her Britannic Majesty's Government of The United Kingdom of Great Britain and N. Ireland

发明人： LEE, Martin, Alan ,  BIRD, Hilary ,  LESLIE, Dario, LyallCBD Porton Down ,  SQUIRRELL, David, James ,  SHAW, JohnCentral Research Laboratories Limited ,  WENN, DavidCentral Research Laboratories Limited ,  DEACON,JulieCentral Research Laboratories Limited

IPC分类号： B01L7/00 ,  B01L3/00 ,  C12Q1/68

CPC分类号： B01L7/52 ,  B01L3/52 ,  B01L3/523

摘要： A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.